Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate

Satoh, Yasuharu; Tajima, Kenji; Nakamoto, Sho; Xuerong, Han; Matsushima, Tokuo; Ohshima, Takeshi; Kawano, Shin; Erata, Tomoki; Dairi, Tohru; Munekata, Masanobu

Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate

Satoh, Yasuharu; Tajima, Kenji; Nakamoto, Sho et al.

Journal of Applied Microbiology, 2011, 111(4), 811-817

Permalink : https://hdl.handle.net/2115/50148

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アクセス権	open access
URI	https://hdl.handle.net/2115/50148
タイトル	Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate
著者	Satoh, Yasuharu ; Tajima, Kenji ; Nakamoto, Sho ; Xuerong, Han ; Matsushima, Tokuo ; Ohshima, Takeshi ; Kawano, Shin ; Erata, Tomoki ; Dairi, Tohru ; Munekata, Masanobu
e-Rad_Researcher 30360928 著者名 Satoh, Yasuharu
e-Rad_Researcher 00271643 著者名 Tajima, Kenji
著者名 Nakamoto, Sho
著者名 Xuerong, Han
著者名 Matsushima, Tokuo
著者名 Ohshima, Takeshi
著者名 Kawano, Shin
e-Rad_Researcher 30213581 著者名 Erata, Tomoki
e-Rad_Researcher 70264679 著者名 Dairi, Tohru
e-Rad_Researcher 50261326 著者名 Munekata, Masanobu
言語	英語
キーワード	biodiesel fuel by-product
	polyhydroxyalkanoate
	pseudomonas
	thermotolerant bacterium
発行日	2011-10
出版者	Blackwell Publishing
収録物名	Journal of Applied Microbiology
巻(号)	111(4)
ページ	811-817
抄録	Aims: The aim of the present study was to isolate a thermotolerant microorganism that produces polyhydroxyalkanoates (PHAs) composed of medium-chain-length (mcl)-HA units from a biodiesel fuel (BDF) by-product as a carbon source. Methods and Results: We successfully isolated a thermotolerant microorganism, strain SG4502, capable to accumulate mcl-PHA from a BDF by-product as a carbon source at a cultivation temperature of 45℃. The strain could also produce mcl-PHA from acetate, octanoate, and dodecanoate as sole carbon sources at cultivation temperatures up to 55℃. Taxonomic studies and 16S rRNA gene sequence analysis revealed that strain SG4502 was phylogenetically affiliated with species of the genus Pseudomonas. This study is the first report of PHA synthesis by a thermotolerant Pseudomonas. Conclusions: A novel thermotolerant bacterium capable to accumulate mcl-PHA from a BDF by-product was successfully isolated. Significance and Impact of the Study: A major issue regarding industrial production of microbial PHAs is their much higher production cost compared with conventional petrochemical-based plastic materials. Especially significant are the cost of a fermentative substrate and the running cost to maintain a temperature suitable for microbial growth. Thus, strain SG4502, isolated in this study, which assimilates BDF by-product and produces PHA at high temperature, would be very useful for practical application in industry.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The definitive version is available at www.blackwell-synergy.com
PISSN	1364-5072
関連情報	PMID 21714837
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/j.1365-2672.2011.05093.x