Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal

Poudel, Ajay; Nakajima, Chie; Fukushima, Yukari; Suzuki, Haruka; Pandey, Basu Dev; Maharjan, Bhagwan; Suzuki, Yasuhiko

Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal

Poudel, Ajay; Nakajima, Chie; Fukushima, Yukari et al.

Antimicrobial Agents and Chemotherapy, 2012, 56(6), 2831-2836

Permalink : https://hdl.handle.net/2115/50780

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アクセス権	open access
URI	https://hdl.handle.net/2115/50780
タイトル	Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal
著者	Poudel, Ajay ; Nakajima, Chie ; Fukushima, Yukari ; Suzuki, Haruka ; Pandey, Basu Dev ; Maharjan, Bhagwan ; Suzuki, Yasuhiko
著者名 Poudel, Ajay
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
著者名 Fukushima, Yukari
著者名 Suzuki, Haruka
著者名 Pandey, Basu Dev
著者名 Maharjan, Bhagwan
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Multi drug-resistant
	Mycobacterium tuberculosis
	rpoB
	katG
	inhA
発行日	2012-06
出版者	American Society for Microbiology
収録物名	Antimicrobial Agents and Chemotherapy
巻(号)	56(6)
ページ	2831-2836
抄録	Despite being one of the first countries globally to introduce multidrug-resistant tuberculosis (MDR-TB) case management, the number of MDR-TB cases is continuing to rise in Nepal. Rapid molecular tests applicable in this setting to identify resistant organisms would be an effective tool in reversing this trend. To develop such tools, information about the frequency and distribution of mutations that are associated with phenotypic drug resistance in Mycobacterium tuberculosis is required. In the present study, we investigated the prevalence of mutations in rpoB and katG genes and the inhA promoter region in 158 M. tuberculosis isolates, 109 phenotypically MDR and 49 non-MDR collected in Nepal, by DNA sequencing. Mutations affecting the 81-bp rifampicin (RIF) resistance-determining region (RRDR) of rpoB were identified in 106 of 109 (97.3%) RIF-resistant isolates. The codons most frequently affected were 531, 526 and 516 with percentages of 58.7%, 15.6% and 15.6%, respectively. Of 113 isoniazid (INH)-resistant isolates, 99 (87.6%) had mutations in the katG gene, with Ser315Thr being the most prevalent (81.4%) substitution. Mutations in the inhA promoter region were detected in 14 (12.4%) INH-resistant isolates. The results from this study provide an overview of the current situation of RIF and INH resistance in M. tuberculosis in Nepal and can serve as a basis for developing or improving rapid molecular-based tests to monitor drug-resistant strains in this country.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	© 2012 American Society for Microbiology
PISSN	0066-4804
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1128/AAC.06418-11