A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose

Kim, Young-Min; Saburi, Wataru; Yu, Shukun et al.

Journal of Biological Chemistry, 2012, 287(27), 22441-22444

Permalink : https://hdl.handle.net/2115/51767

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アクセス権
open access
URI
https://hdl.handle.net/2115/51767
タイトル
A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose
著者
Kim, Young-Min ; Saburi, Wataru ; Yu, Shukun ; Nakai, Hiroyuki ; Maneesan, Janjira ; Kang, Min-Sun ; Chiba, Seiya ; Kim, Doman ; Okuyama, Masayuki ; Mori, Haruhide ; Kimura, Atsuo
著者名
Kim, Young-Min
e-Rad_Researcher
00598089
著者名
Saburi, Wataru
著者名
Yu, Shukun
著者名
Nakai, Hiroyuki
著者名
Maneesan, Janjira
著者名
Kang, Min-Sun
e-Rad_Researcher
30001449
著者名
Chiba, Seiya
著者名
Kim, Doman
e-Rad_Researcher
00344490
著者名
Okuyama, Masayuki
e-Rad_Researcher
80241363
著者名
Mori, Haruhide
e-Rad_Researcher
90186312
著者名
Kimura, Atsuo
言語
英語
キーワード
α-glucosidase
hydration
trans-addition
1,5-anhydrofructose
発行日
2012-06-29
出版者
American Society for Biochemistry and Molecular Biology
収録物名
Journal of Biological Chemistry
巻(号)
287(27)
ページ
22441-22444
抄録
α-Glucosidase is in the glycoside hydrolase family 13 (13AG) and 31 (31AG). Only 31AGs can hydrate the D-glucal double bond to form α-2-deoxyglucose. Since 1,5-anhydrofructose (AF), having 2-OH-group, mimics the oxocarbenium-ion transition-state, AF may be a substrate for α-glucosidases. α-Glucosidase-catalyzed hydration produced α-glucose from AF, which plateaued with time. Combined reaction with α-1,4-glucan lyase (GL) and 13AG eliminated the plateau. Aspergillus niger α-glucosidase (31AG), which is stable in organic solvent, produced ethyl α-glucoside from AF in 80% ethanol. The findings indicate that α-glucosidases catalyze trans-addition. This is the first report of α-glucosidase-associated glucose formation from AF, possibly contributing to salvage pathway of un-utilized AF.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This research was originally published in Journal of Biological Chemistry. Young-Min Kim; Wataru Saburi; Shukun Yu; Hiroyuki Nakai; Janjira Maneesan; Min-Sun Kang; Seiya Chiba; Doman Kim; Masayuki Okuyama; Haruhide Mori; Atsuo Kimura. A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose. Journal of Biological Chemistry. 2012; 287:22441-22444. © the American Society for Biochemistry and Molecular Biology.
PISSN
0021-9258
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1074/jbc.C112.360909