Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina

Maeda, Takashi; Kawai, Tadashi; Nakaoka, Masahiro; Yotsukura, Norishige

Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina

Maeda, Takashi; Kawai, Tadashi; Nakaoka, Masahiro et al.

Journal of Applied Phycology, 2013, 25(1), 337-347

Permalink : https://hdl.handle.net/2115/52032

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/52032
タイトル	Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina
著者	Maeda, Takashi ; Kawai, Tadashi ; Nakaoka, Masahiro ; Yotsukura, Norishige
著者名 Maeda, Takashi
著者名 Kawai, Tadashi
著者名 Nakaoka, Masahiro
e-Rad_Researcher 60312344 著者名 Yotsukura, Norishige
言語	英語
キーワード	AFLP
	DNA extraction
	DNA purification
	Fragment analysis
	Saccharina
	Phaeophyta
発行日	2013-02
出版者	Springer Netherlands
収録物名	Journal of Applied Phycology
巻(号)	25(1)
ページ	337-347
抄録	The DNA fragment analysis can become an effective tool to study genetic differences between not only species but also individuals on saccharinan kelp from which the little genetic diversity was reported. This time, extraction methods of suitable DNA for use in the analysis with a capillary sequencer was examined on Saccharina japonica var. diabolica that contains polysaccharide abundantly. When AFLP was performed using genomic DNA extracted by seven different methods: (1) commercial kit, (2) original CTAB method, (3)-(5) three types of modified CTAB method, (6) modified SDS method, (7) combination of CTAB method and SDS method, high reproducible peak that was worth for the analysis was noticeable in the electropherogram in the experiment with the last combination method (7). It is considered that the pretreatment washing of polysaccharide and the subsequent purification for protein and RNA in SDS method and for polysaccharide in CTAB method are effective to obtain the high purity DNA.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at www.springerlink.com
PISSN	0921-8971
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10811-012-9868-3