Rapid species identification of fresh and processed scallops by multiplex PCR

Marín, Alan; Fujimoto, Takafumi; Arai, Katsutoshi

Food control, 2013, 32(2), 472-476

Permalink : https://hdl.handle.net/2115/52047

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ファイル	フォーマット	サイズ	閲覧回数	説明
RevisedManuscript	pdf	9.06 MB	2,401

アクセス権	open access
URI	https://hdl.handle.net/2115/52047
タイトル	Rapid species identification of fresh and processed scallops by multiplex PCR
著者	Marín, Alan ; Fujimoto, Takafumi ; Arai, Katsutoshi
著者名 Marín, Alan
著者名 Fujimoto, Takafumi
e-Rad_Researcher 00137902 著者名 Arai, Katsutoshi
言語	英語
キーワード	Pectinidae
	Scallop
	Food control
	16S rRNA gene
	Multiplex PCR
発行日	2013-08
出版者	Elsevier
収録物名	Food control
巻(号)	32(2)
ページ	472-476
抄録	Food control policies regarding to seafood label authenticity have become a global issue due to increased incidence of species substitution or mislabelling. Proper species-level identification in processed scallop products is hindered by the lack of morphological characters such as their valves. In order to identify four commercially important scallop species (Argopecten purpuratus, A. irradians, Mizuhopecten yessoensis, Pecten albicans) a species-specific multiplex PCR reaction is described herein. Novel reverse species-specific primers in combination with one universal forward primer designed to amplify a partial region of the mitochondrial 16S rRNA gene were assayed in fresh as well as in manufactured scallop samples. All PCR reactions showed a high specificity allowing an unambiguous species authentication.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0956-7135
NCID	AA1082764X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.foodcont.2013.01.007