open access

https://hdl.handle.net/2115/52078

Molecular Species of the Enzymatically Synthesized Polyunsaturated Fatty Acid Rich Triglyceride

日本語

1991

日本水産学会

Bulletin of the Japanese Society of Scientific Fisheries

57(1)

119-125

Enzymatic glyceride synthesis and acid interchange using icosapentaenoic acid (EPA), docosahexaenoic acid (DHA), γ-linolenic acid (GLA), and EPA and DHA concentrated saponified fatty acid mixture obtained from sardine oil were carried out through the use of four kinds of microbial lipases. Lipase TOYO (Chromobacterium viscosum) was the most effective enzyme for glyceride synthesis as well as acid interchange of triglyceride (TG) rich in polyunsaturated fatty acids. There was a general tendency, except with Lipase TOYO, that DHA was difficult to be incorporated into a glycerol molecule compared to the other fatty acids. Fatty acid composition of TG synthesized from a highly concentrated fatty acid mixture (EPA+DHA: 68.8%) by the use of Lipase TOYO and Lipase OF (Candida cylindracea), directly reflected that of the substrate fatty acid flixture (i.e. EPA+DHA was more than 65%). And the TG molecular species having combination of only EPA and DHA such as (EPA, EPA, EPA), (EPA, EPA, DHA), (EPA, DHA, DHA), and (DHA, DHA, DHA) were dominant accounting for the half of the obtained TG.

学術雑誌論文

Version of Record

© 1990 The Japanese Society of Fisheries Science

0021-5392

1349-998X