open access

https://hdl.handle.net/2115/52081

Synthesis of Phosphatidylcholine Containing Highly Unsaturated Fatty Acid by Phospholipase A2 and Effect on Retinoic Acid Induced Differentiation of HL-60 Cells

日本語

1993

日本水産学会

Bulletin of the Japanese Society of Scientific Fisheries

59(2)

309-314

Phosphatidylcholine containing highly unsaturated fatty acid (HUFA-PC) was prepared by porcine pancreatic phospholipase A2, which catalyzed esterification between lysophosphatidylcholine (LPC) and highly unsaturated fatty acid (HUFA), under a scaled-up reaction system. Fatty acid mixture prepared from sardine oil, purified eicosapentaenoic acid (EPA), and purified docosahexaenoic acid (DHA) were used as the substrates of HUFA. The yield of HUFA-PC was 17.0-19.9%. Synthesized phosphatidylcholine containing DHA (DHA-PC) prepared from LPC and DHA had a DHA content of 46.5%, which indicated almost the maximum theoretical incorporation rate. DHA-PC molecular species consisted of (16:0, DHA), (18:0, DHA), (18:1, DHA), (18:2, DHA), and (18:3, DHA). It was obvious that the acyl groups in sn-1 of these molecular species originated from the substrate LPC. The rate of retinoic acid induced differentiation of the human promyelocytic leukemia cell line (HL-60) was promoted by growing HL-60 cells in 10% FBS-PNM pre-treated with either EPA-PC or DHA-PC.

学術雑誌論文

Version of Record

© 1993 The Japanese Society of Fisheries Science

0021-5392

1349-998X