open access

https://hdl.handle.net/2115/52098

Application of an alginate lyase from Alteromonas sp. for isolation of protoplasts from a brown algae Laminaria japonica

英語

1993

日本水産学会

Bulletin of the Japanese Society of Scientific Fisheries

59(4)

705-709

An alginate lyase from Alteromonas sp. H-4 was used for the preparation of protoplast from young sporophytes of Laminaria japonica. Optimum conditions for the isolation of protoplasts were as follows: a) fine pieces of the sporophytes chopped by a razor were incubated in an enzyme mixture for 3 to 5h at 15°C with shaking, b) the composition of the enzyme mixture was purified alginate lyase (30U/ml) with cellulase Onozuka R-10 (1.5%) in a hypertonic solution (0.7M mannitol, 25mM MgCl2, and 5mM HEPES in 50% seawater, pH7.8). Yields of protoplast were 106-107 cells/g (sporophytes) and protoplast viability was more than 75%. Protoplasts were brown-greenish and spherical with a diameter of 10-25μm. The purity of alginate lyase from the strain H-4 scarcely influenced the yield of protoplast, but purified enzyme produced the highest viability of cells.

学術雑誌論文

Version of Record

© 1993 The Japanese Society of Fisheries Science

0021-5392

1349-998X