open access

https://hdl.handle.net/2115/52101

Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production

英語

Polyhydroxybutyrate

2012-03

Springer-Verlag

Applied Microbiology and Biotechnology

93(5)

1917-1925

The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli. Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA) and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by β-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester.

学術雑誌論文

Accepted Manuscript

The original publication is available at www.springerlink.com

0175-7598