Draft Genome Sequence of a Psychrotolerant Sulfur-Oxidizing Bacterium, Sulfuricella denitrificans skB26, and Proteomic Insights into Cold Adaptation

Watanabe, Tomohiro; Kojima, Hisaya; Fukui, Manabu

Draft Genome Sequence of a Psychrotolerant Sulfur-Oxidizing Bacterium, Sulfuricella denitrificans skB26, and Proteomic Insights into Cold Adaptation

Watanabe, Tomohiro; Kojima, Hisaya; Fukui, Manabu

Applied and Environmental Microbiology, 2012, 78(18), 6545-6549

Permalink : https://hdl.handle.net/2115/52110

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Supplemental_file_5	xls	20.0 KB	314	Supplemental material (Table S4)
Supplemental_file_4	xls	60.0 KB	386	Supplemental material (Table S3)
Supplemental_file_3	xls	31.0 KB	341	Supplemental material (Table S2)
Supplemental_file_2	xls	34.0 KB	353	Supplemental material (Table S1)
Supplemental_file_1	pdf	23.2 KB	572	Supplemental material (Fig. S1)
AEM78-18_6545-6549	pdf	646 KB	1,103

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/52110
タイトル	Draft Genome Sequence of a Psychrotolerant Sulfur-Oxidizing Bacterium, Sulfuricella denitrificans skB26, and Proteomic Insights into Cold Adaptation
著者	Watanabe, Tomohiro ; Kojima, Hisaya ; Fukui, Manabu
著者名 Watanabe, Tomohiro
e-Rad_Researcher 70400009 著者名 Kojima, Hisaya
e-Rad_Researcher 60305414 著者名 Fukui, Manabu
言語	英語
発行日	2012-09
出版者	American Society for Microbiology
収録物名	Applied and Environmental Microbiology
巻(号)	78(18)
ページ	6545-6549
抄録	Except for several conspicuous cases, very little is known about sulfur oxidizers living in natural freshwater environments. Sulfuricella denitrificans skB26 is a psychrotolerant sulfur oxidizer recently isolated from a freshwater lake, as a representative of a new genus in the class Betaproteobacteria. In this study, approximately 3.2 Mb of draft genome sequence of the strain skB26 was obtained. In the draft genome consisting of 23 contigs, a single rRNA operon, 43 tRNA genes, and 3,133 coding sequences were identified. The identified genes include those required for sulfur oxidation, denitrification, and carbon fixation. Comparative proteomic analysis was conducted to assess cold adaptation mechanisms of this organism. From cells grown at 22℃ and 5℃, proteins were extracted for analysis with nano-liquid chromatography-electrospray ionization-tandem mass spectrometry. In the cells cultured at 5℃, relative abundances of ribosomal proteins, cold shock proteins, and DEAD/DEAH box RNA helicases were increased in comparison to 22℃. These results suggest that maintenance of proper translation is critical for growth under low-temperature conditions, similar to other cold-adapted prokaryotes.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	© 2012 American Society for Microbiology
PISSN	0099-2240
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1128/AEM.01349-12