Effects of endogenous proteins and microRNA target sequence in a positive feedback system.

Kanda, Genki N; Togashi, Ryohei; Harashima, Hideyoshi; Kamiya, Hiroyuki

Effects of endogenous proteins and microRNA target sequence in a positive feedback system.

Kanda, Genki N; Togashi, Ryohei; Harashima, Hideyoshi et al.

Biological & pharmaceutical bulletin, 2012, 35(9), 1534-1538

Permalink : https://hdl.handle.net/2115/52122

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/52122
タイトル	Effects of endogenous proteins and microRNA target sequence in a positive feedback system.
著者	Kanda, Genki N ; Togashi, Ryohei ; Harashima, Hideyoshi ; Kamiya, Hiroyuki
著者名 Kanda, Genki N
著者名 Togashi, Ryohei
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
e-Rad_Researcher 10204629 著者名 Kamiya, Hiroyuki
言語	英語
キーワード	positive feedback system
	secreted alkaline phosphatase
	transcription factor
	microRNA
	long-term transgene expression
発行日	2012-09
出版者	Pharmaceutical Society of Japan
収録物名	Biological & pharmaceutical bulletin
巻(号)	35(9)
ページ	1534-1538
抄録	A positive feedback system, using GAL4-vp16 (a fusion protein of yeast GAL4 and herpes simplex virus vp16) as an activator and firefly luciferase as a reporter, maintained luciferase expression for 7 d in mice. However, the luciferase expression decreased after 7 d, and this phenomenon could be caused by immunoreactions against these exogenous proteins. This hypothesis was examined by the following three strategies, designed to avoid the putative immunoreactions: (i) use of the endogenous secreted alkaline phosphatase (SEAP) protein as a reporter, (ii) replacement of vp16 with endogenous transcription factors, and (iii) insertion of the target sequence of microRNA expressed in cells of hematopoietic origin, to suppress GAL4-vp16 expression in antigen-presenting cells. The results obtained in this study suggested that silencing would be induced by mechanism(s) besides immunoreactions against reporter and activator proteins.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1347-5215
関連情報	PMID 22975505
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1248/bpb.b12-00329