Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631

Sasaki, Keisuke; Yoneda, Akihiro; Ninomiya, Akinori; Kawahara, Manabu; Watanabe, Tomomasa

Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631

Sasaki, Keisuke; Yoneda, Akihiro; Ninomiya, Akinori et al.

Biochemical and Biophysical Research Communications, 2013, 430(1), 161-166

Permalink : https://hdl.handle.net/2115/52138

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/52138
タイトル	Both antiviral activity and intracellular localization of chicken Mx protein depend on a polymorphism at amino acid position 631
著者	Sasaki, Keisuke ; Yoneda, Akihiro ; Ninomiya, Akinori ; Kawahara, Manabu ; Watanabe, Tomomasa
著者名 Sasaki, Keisuke
e-Rad_Researcher 00451419 著者名 Yoneda, Akihiro
著者名 Ninomiya, Akinori
e-Rad_Researcher 70468700 著者名 Kawahara, Manabu
e-Rad_Researcher 10100174 著者名 Watanabe, Tomomasa
言語	英語
キーワード	Mx
	Chicken
	Polymorphism
	Vesicular stomatitis virus
	Intracellular localization
発行日	2013-01-04
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	430(1)
ページ	161-166
抄録	The Mx protein is known to inhibit the multiplication of several RNA viruses. In chickens, a polymorphism at amino acid position 631 (631 aa) of Mx protein has been suggested to be involved in the antiviral ability against vesicular stomatitis virus (VSV) and influenza virus, indicating that a Ser-to-Asn substitution at 631 aa is the source of this antiviral ability. However, how the substitution at 631 aa contributes to the antiviral activity remains to be clarified. In this study, we investigated differences in antiviral activity against VSV and intracellular localization between Ser and Asn types at 631 aa of the chicken Mx protein. The results showed that chicken Mx protein with an Asn at 631 aa inhibited VSV multiplication and Mx distribution in a granular-like pattern in the cytoplasm. However, Mx carrying the Ser type did not inhibit viral growth and homogenous spread throughout the cytoplasm. Furthermore, we found that replacing Ser with Asn at 631 aa provided Mx with antiviral activity against VSV, with Mx showing granular-like distribution in the cytoplasm. These results demonstrated that a single amino acid polymorphism at 631 aa of the chicken Mx protein altered both the antiviral activity and intracellular localization.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 23201406
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2012.11.053