Expression of vascular endothelial growth factor C in human pterygium

Fukuhara, Junichi; Kase, Satoru; Ohashi, Tsutomu; Ando, Ryo; Dong, Zhenyu; Noda, Kousuke; Ohguchi, Takeshi; Kanda, Atsuhiro; Ishida, Susumu

Expression of vascular endothelial growth factor C in human pterygium

Fukuhara, Junichi; Kase, Satoru; Ohashi, Tsutomu et al.

Histochemistry and Cell Biology, 2013, 139(2), 381-389

Permalink : https://hdl.handle.net/2115/52139

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アクセス権	open access
URI	https://hdl.handle.net/2115/52139
タイトル	Expression of vascular endothelial growth factor C in human pterygium
著者	Fukuhara, Junichi ; Kase, Satoru ; Ohashi, Tsutomu ; Ando, Ryo ; Dong, Zhenyu ; Noda, Kousuke ; Ohguchi, Takeshi ; Kanda, Atsuhiro ; Ishida, Susumu
著者名 Fukuhara, Junichi
e-Rad_Researcher 60374394 著者名 Kase, Satoru
著者名 Ohashi, Tsutomu
著者名 Ando, Ryo
著者名 Dong, Zhenyu
e-Rad_Researcher 90296666 著者名 Noda, Kousuke
著者名 Ohguchi, Takeshi
著者名 Kanda, Atsuhiro
e-Rad_Researcher 10245558 著者名 Ishida, Susumu
言語	英語
キーワード	VEGF-C
	VEGFR-3
	Lymphangiogenesis
	Lymphatic vessel density
	Pterygium
発行日	2013-02
出版者	Springer-Verlag
収録物名	Histochemistry and Cell Biology
巻(号)	139(2)
ページ	381-389
抄録	Purpose: Vascular endothelial growth factor C (VEGF-C) and its receptor VEGFR-3 mediate lymphangiogenesis. In this study, we analyzed the expression of VEGF-C and VEGFR-3 as well as lymphatic vessels in the pterygium and normal conjunctiva of humans. Methods: Fifteen primary nasal pterygia and three normal bulbar conjunctivas, surgically removed, were examined in this study. The lymphatic vessel density (LVD) and blood vessel density (BVD) were determined by the immunolabeling of D2-40 and CD31, markers for lymphatic and blood vessels, respectively. VEGF-C and VEGFR-3 expression in pterygial and conjunctival tissue proteins was detected by Western blotting. Expressions of VEGF-C and VEGFR-3 were evaluated using immunohistochemistry. Results: The LVD was significantly higher in the pterygium than normal conjunctiva (p<0.05). Western blot demonstrated high-level expression of VEGF-C and VEGFR-3 in the pterygium compared with normal conjunctiva. VEGF-C immunoreactivity was detected in the cytoplasm of pterygial and normal conjunctival epithelial cells. The number of VEGF-C-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival cells (p<0.05). VEGFR-3 immunoreactivity was localized in the D2-40-positive lymphatic endothelial cells. Conclusions: The present findings suggest the potential role of VEGF-C in the pathogenesis and development of a pterygium through lymphangiogenesis and the VEGF-C/VEGFR-3 pathway as a novel therapeutic target for the human pterygium.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at www.springerlink.com
PISSN	0948-6143
関連情報	PMID 22910845
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00418-012-1019-z