Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice

Lennikov, Anton; Kitaichi, Nobuyoshi; Kase, Satoru; Noda, Kousuke; Horie, Yukihiro; Nakai, Akira; Ohno, Shigeaki; Ishida, Susumu

Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice

Lennikov, Anton; Kitaichi, Nobuyoshi; Kase, Satoru et al.

International Journal of Molecular Sciences, 2013, 14(1), 2175-2189

Permalink : https://hdl.handle.net/2115/52140

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アクセス権	open access
URI	https://hdl.handle.net/2115/52140
タイトル	Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice
著者	Lennikov, Anton ; Kitaichi, Nobuyoshi ; Kase, Satoru ; Noda, Kousuke ; Horie, Yukihiro ; Nakai, Akira ; Ohno, Shigeaki ; Ishida, Susumu
著者名 Lennikov, Anton
e-Rad_Researcher 40431366 著者名 Kitaichi, Nobuyoshi
e-Rad_Researcher 60374394 著者名 Kase, Satoru
e-Rad_Researcher 90296666 著者名 Noda, Kousuke
著者名 Horie, Yukihiro
著者名 Nakai, Akira
e-Rad_Researcher 50002382 著者名 Ohno, Shigeaki
e-Rad_Researcher 10245558 著者名 Ishida, Susumu
言語	英語
キーワード	GGA
	geranylgeranylacetone
	HSP
	HSP70
	UVB
	keratitis
	cornea
	apoptosis
発行日	2013-01
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	14(1)
ページ	2175-2189
抄録	Acute ultraviolet (UV) B exposure causes photokeratitis and induces apoptosis in corneal cells. Geranylgeranylacetone (GGA) is an acyclic polyisoprenoid that induces expression of heat shock protein (HSP) 70, a soluble intracellular chaperone protein expressed in various tissues, protecting cells against stress conditions. We examined whether induction of HSP70 has therapeutic effects on UV-photokeratitis in mice. C57 BL/6 mice were divided into four groups, GGA-treated (500 mg/kg/mouse) and UVB-exposed (400 mJ/cm2), GGA-untreated UVB-exposed (400 mJ/cm2), GGA-treated (500 mg/kg/mouse) but not exposed and naive controls. Eyeballs were collected 24 h after irradiation, and corneas were stained with hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). HSP70, reactive oxygen species (ROS) production, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and protein kinase B (Akt) expression were also evaluated. Irradiated corneal epithelium was significantly thicker in the eyes of mice treated with GGA compared with those given the vehicle alone (p < 0.01). Significantly fewer TUNEL-positive cells were observed in the eyes of GGA-treated mice than controls after irradiation (p < 0.01). Corneal HSP70 levels were significantly elevated in corneas of mice treated with GGA (p < 0.05). ROS signal was not affected by GGA. NF-κB activation was reduced but phospho-(Ser/Ther) Akt substrate expression was increased in corneas after irradiation when treated with GGA. GGA-treatment induced HSP70 expression and ameliorated UV-induced corneal damage through the reduced NF-κB activation and possibly increased Akt phosphorilation.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 3.0 Unported
PISSN	1422-0067
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms14012175