open access

https://hdl.handle.net/2115/52255

Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids

英語

endosome

2013-03

American Society for Biochemistry and Molecular Biology

Journal of Lipid Research

54(3)

831-842

Very long-chain fatty acids (VLCFAs), fatty acids with chain length of > 20, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the presented study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and identified the VPS21 gene, encoding a Rab GTPase, as such a suppressor. When the vps21Δ mutation was introduced into a deletion mutant of the SUR4 gene, which encodes a VLCFA elongase, a synthetic growth defect was observed. Endosome-mediated vesicular trafficking pathways, including endocytosis and the CPY pathway, were severely impaired in sur4Δ vps21Δ double mutants, while the AP-3 pathway that bypasses the endosome was unaffected. In addition, the sur4Δ mutant also exhibited a synthetic growth defect when combined with the deletion of VPS3, which encodes a subunit of the CORVET (class C core vacuole/endosome tethering) complex that tethers transport vesicles to the late endosome/multivesicular body (MVB). These results suggest that requirement of VLCFAs is especially high in the endosomal pathways, of all the intracellular trafficking pathways.

学術雑誌論文

Accepted Manuscript

Copyright © 2013 the American Society for Biochemistry and Molecular Biology

0022-2275