Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses

Okamatsu, Masatoshi; Feng, Fei; Ohyanagi, Tatsuya; Nagahori, Noriko; Someya, Kazuhiko; Sakoda, Yoshihiro; Miura, Nobuaki; Nishimura, Shin-Ichiro; Kida, Hiroshi

Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses

Okamatsu, Masatoshi; Feng, Fei; Ohyanagi, Tatsuya et al.

Journal of Virological Methods, 2013, 187(2), 390-394

Permalink : https://hdl.handle.net/2115/52274

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アクセス権	open access
URI	https://hdl.handle.net/2115/52274
タイトル	Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses
著者	Okamatsu, Masatoshi ; Feng, Fei ; Ohyanagi, Tatsuya ; Nagahori, Noriko ; Someya, Kazuhiko ; Sakoda, Yoshihiro ; Miura, Nobuaki ; Nishimura, Shin-Ichiro ; Kida, Hiroshi
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
著者名 Feng, Fei
著者名 Ohyanagi, Tatsuya
e-Rad_Researcher 90372268 著者名 Nagahori, Noriko
著者名 Someya, Kazuhiko
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
e-Rad_Researcher 80372267 著者名 Miura, Nobuaki
e-Rad_Researcher 00183898 著者名 Nishimura, Shin-Ichiro
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
言語	英語
キーワード	Influenza virus
	Hemagglutinin
	Fluorescence polarization
	Quantum dot
	High-throughput screening
発行日	2013-02
出版者	Elsevier B.V.
収録物名	Journal of Virological Methods
巻(号)	187(2)
ページ	390-394
抄録	Attachment of influenza virus to susceptible cells is mediated by viral protein hemagglutinin (HA), which recognizes cell surface glycoconjugates that terminate in α-sialosides. To develop anti-influenza drugs based on inhibition of HA-mediated infection, novel fluorescent nanoparticles displaying multiple biantennary N-glycan chains with α-sialosides (A2-PC-QDs) that have high affinity for the HA were designed and constructed. The A2-PC-QDs enabled an easy and efficient fluorescence polarization (FP) assay for detection of interaction with the HA and competitive inhibition even by small molecule compounds against A2-PC-QDs-HA binding. The quantum dot (QD)-based FP assay established in the present study is a useful tool for high-throughput screening and to accelerate the development of novel and more effective blockers of the viral attachment of influenza virus.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0166-0934
関連情報	PMID 23164994
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jviromet.2012.11.004