open access

https://hdl.handle.net/2115/52356

Effect of gas adding on the preservation of dissociated neonatal rat cardiomyocytes at room temperatures

日本語

2013-03-31

Institute of Low Temperature Science, Hokkaido University

Low Temperature Science

71

81-89

If we can preserve the cells, we contribute significantly to the industrial demands by the stable supply of a number of experimental samples from one individual. However, some cells exist in high demand although it is difficult to preserve, such as the cardiomyocytes that make up the heart. In this study, we have examined the preservation technique by applying gases to rise the storage temperature limit of the dispersed primary rat cardiomyocytes. We evaluated the health of its functions under physiological conditions by culturing the cells after the storage by applying 0.5MPa gases for 72 hours at temperatures ranged from 9.4 to 35℃. As a result, we observed no difference between the preserved cells with and without applying inert gas, nitrogen gas and hydrocarbon gas in the present experimental conditions. However, we found the significant negative effect by applying the mixed gas containing oxygen during the culture test although the dispersed condition was kept during the preservation period at higher temperature. It is considered that the dissolved oxygen would inhibit the adhesion function of cardiomyocytes.

紀要論文

Version of Record

1880-7593