open access

https://hdl.handle.net/2115/52570

Towards intergeneric hybridization between Alstroemeria L. and Bomarea Mirb.

英語

pollen tube

2012-12

Global Science Books

Floriculture and Ornamental Biotechnology

6(2)

146-149

There are many interspecific hybrids of Alstroemeria. In this study, the possibility of intergeneric hybridization between Alstroemeria and Bomarea Mirb. was examined through the development of pollination procedures and ovule culture based on the histological observation of embryo and endosperm development after intergeneric pollination. Three methods of pollination (stigmatic, cut-style, and non-style) were combined with four different pollen types (fresh, frozen, non-germinated, and pre-germinated). We observed that the pollen tubes of Bomarea coccinea (Ruiz & Pav.) Baker could reach to the ovules of Alstroemeria aurea Graham 48 hours after stigmatic pollination with frozen pollen. Histological observations revealed that a primary embryo was formed, but subsequently aborted during development. This study demonstrates the possibility of intergeneric hybridization between Alstroemeria and Bomarea, but showed that there are post-fertilization barriersbetween A. pelegrina and B. coccinea. Further study is needed to investigate the optimum conditions for obtaining hybrid progeny.

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© 2012 Global Science Books

1749-0294