Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney

Yamane, Takuya; Hachisu, Rei; Yuguchi, Motoki; Takeuchi, Keisuke; Murao, Sato; Yamamoto, Yoshio; Ogita, Hisakazu; Takasawa, Toshihide; Ohkubo, Iwao; Ariga, Hiroyoshi

Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney

Yamane, Takuya; Hachisu, Rei; Yuguchi, Motoki et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 430(2), 482-487

Permalink : https://hdl.handle.net/2115/52625

このアイテムのアクセス数:1,289件（2026-08-26 07:16 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Manuscript with Title Page-1	pdf	362 KB	1,234

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/52625
タイトル	Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney
著者	Yamane, Takuya ; Hachisu, Rei ; Yuguchi, Motoki ; Takeuchi, Keisuke ; Murao, Sato ; Yamamoto, Yoshio ; Ogita, Hisakazu ; Takasawa, Toshihide ; Ohkubo, Iwao ; Ariga, Hiroyoshi
著者名 Yamane, Takuya
著者名 Hachisu, Rei
著者名 Yuguchi, Motoki
著者名 Takeuchi, Keisuke
著者名 Murao, Sato
著者名 Yamamoto, Yoshio
著者名 Ogita, Hisakazu
著者名 Takasawa, Toshihide
著者名 Ohkubo, Iwao
e-Rad_Researcher 20143505 著者名 Ariga, Hiroyoshi
言語	英語
キーワード	Legumain
	Annexin A2
	Cationic liposome
	siRNA
	In vivo
	Mouse kidney
発行日	2013-01-11
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
収録物名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
巻(号)	430(2)
ページ	482-487
抄録	Legumain (EC 3.4.22.34) is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro. In this study, to determine whether annexin A2 is cleaved by legumain in vivo, siRNA-lipoplex targeting mouse legumain was injected into mouse tail veins. Mouse kidneys were then isolated and the effect of knockdown of legumain expression on annexin A2 cleavage was examined. The results showed that both legumain mRNA and protein expression levels were decreased in the siRNA-treated mouse kidneys and that legumain activity toward a synthetic substrate, Z-Ala-Ala-Asn-MCA, was decreased by about 40% in the kidney but not in the liver or spleen. Furthermore, cleavage of annexin A2 at the N-terminal region was decreased in the mouse kidney that had been treated with the legumain siRNA-lipoplex. These results suggest that legumain siRNA was delivered to the kidney by using LipoTrust and that the reduced legumain expression inhibited legumain-induced degradation of annexin A2 in vivo. (C) 2012 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 23237799
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2012.12.010