Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain

Ishihara, Seiichiro; Yasuda, Motoaki; Nishioka, Takeshi; Mizutani, Takeomi; Kawabata, Kazushige; Shirato, Hiroki; Haga, Hisashi

Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain

Ishihara, Seiichiro; Yasuda, Motoaki; Nishioka, Takeshi et al.

FEBS Letters, 2013, 587(6), 732-736

Permalink : https://hdl.handle.net/2115/52653

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アクセス権	open access
URI	https://hdl.handle.net/2115/52653
タイトル	Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
著者	Ishihara, Seiichiro ; Yasuda, Motoaki ; Nishioka, Takeshi ; Mizutani, Takeomi ; Kawabata, Kazushige ; Shirato, Hiroki ; Haga, Hisashi
著者名 Ishihara, Seiichiro
著者名 Yasuda, Motoaki
著者名 Nishioka, Takeshi
著者名 Mizutani, Takeomi
著者名 Kawabata, Kazushige
著者名 Shirato, Hiroki
e-Rad_Researcher 00292045 著者名 Haga, Hisashi
言語	英語
キーワード	3D culture
	Collagen gel
	Irradiation
	Invasion
	Lung adenocarcinoma
	Myosin regulatory light chain
発行日	2013-03-18
出版者	Elsevier
収録物名	FEBS Letters
巻(号)	587(6)
ページ	732-736
抄録	Radiotherapy is one of the major treatment modalities for malignancies. However, cells surviving irradiation often display high levels of invasiveness. This study shows that irradiation-tolerant lung adenocarcinoma demonstrates high invasive capability depending on dephosphorylation of the myosin regulatory light chain (MRLC). In a collagen gel overlay condition, low-invasive subclones of lung adenocarcinoma (A549P-3) showed a round morphology and diphosphorylation of MRLC. In contrast, irradiation-tolerant A549P-3 cells (A549P-3IR) displayed high invasiveness and a lower level of MRLC diphosphorylation. In addition, inhibition of MRLC phosphatase activity decreased the invasive activity. These findings suggest that A549P-3IR cells acquire high invasiveness through MRLC dephosphorylation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
関連情報	PMID 23391761
関連情報	URI http://www.sciencedirect.com/science/article/pii/S0014579313001002
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2013.01.055