Biosynthetic assembly of cytochalasin backbone

Fujii, Ryuya; Minami, Atsushi; Gomi, Katsuya; Oikawa, Hideaki

Biosynthetic assembly of cytochalasin backbone

Fujii, Ryuya; Minami, Atsushi; Gomi, Katsuya et al.

Tetrahedron Letters, 2013, 54(23), 2999-3002

Permalink : https://hdl.handle.net/2115/52893

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アクセス権	open access
URI	https://hdl.handle.net/2115/52893
タイトル	Biosynthetic assembly of cytochalasin backbone
著者	Fujii, Ryuya ; Minami, Atsushi ; Gomi, Katsuya ; Oikawa, Hideaki
著者名 Fujii, Ryuya
著者名 Minami, Atsushi
著者名 Gomi, Katsuya
e-Rad_Researcher 00185175 著者名 Oikawa, Hideaki
言語	英語
キーワード	Cytochalasin
	Biosynthesis
	Polyketide synthase
	Non-ribosomal peptide synthetase
	Fungal metabolite
発行日	2013-06-05
出版者	Elsevier
収録物名	Tetrahedron Letters
巻(号)	54(23)
ページ	2999-3002
抄録	Cytochalasins are an important class of fungal natural products in view of structural diversity and biological activities. Although their biosynthetic studies have been examined extensively, the detailed molecular assembly mechanism remains to be solved. We have succeeded to heterologously express the cytochalasin polyketide synthase-non-ribosomal peptide synthetase (PKS-NRPS) hybrid gene ccsA and the trans-acting enoyl-CoA reductase gene ccsC in Aspergillus oryzae. The resultant transformant produced a novel metabolite possessing the cytochalasin backbone. This established that CcsA is capable of constructing the octaketide connected with phenylalanine in collaboration with CcsC, and that CcsA R domain catalyzes reductive cleavage of the thio-tethered PKS-NRPS product. (C) 2013 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0040-4039
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.tetlet.2013.03.120