Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation

Mizutani, Yukiko; Sun, Hui; Ohno, Yusuke; Sassa, Takayuki; Wakashima, Takeshi; Obara, Mari; Yuyama, Kohei; Kihara, Akio; Igarashi, Yasuyuki

Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation

Mizutani, Yukiko; Sun, Hui; Ohno, Yusuke et al.

Plos One, 2013, 8(6), e67317

Permalink : https://hdl.handle.net/2115/53154

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アクセス権	open access
URI	https://hdl.handle.net/2115/53154
タイトル	Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation
著者	Mizutani, Yukiko ; Sun, Hui ; Ohno, Yusuke ; Sassa, Takayuki ; Wakashima, Takeshi ; Obara, Mari ; Yuyama, Kohei ; Kihara, Akio ; Igarashi, Yasuyuki
著者名 Mizutani, Yukiko
著者名 Sun, Hui
著者名 Ohno, Yusuke
e-Rad_Researcher 20342793 著者名 Sassa, Takayuki
著者名 Wakashima, Takeshi
著者名 Obara, Mari
e-Rad_Researcher 80415546 著者名 Yuyama, Kohei
e-Rad_Researcher 50333620 著者名 Kihara, Akio
e-Rad_Researcher 70091965 著者名 Igarashi, Yasuyuki
言語	英語
発行日	2013-06-27
出版者	Public Library of Science
収録物名	Plos One
巻(号)	8(6)
ページ	e67317
抄録	The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide (CER), comprising an ultra long-chain (ULC) fatty acid (FA) of >= 26 carbons (ULC CER), plays an essential role in barrier formation. ULC acyl-CoAs, produced by the FA elongase ELOVL4, are converted to ULC CERs by the CER synthase CERS3. In the presented study, we observed that ELOVL4 and CERS3 mRNAs increased during keratinocyte differentiation in vivo and in vitro. We also determined that peroxisome proliferator-activated receptor beta/delta is involved in the up-regulation of the mRNAs. Knockdown of CERS3 caused a reduction in the elongase activities toward ULC acyl-CoAs, suggesting that CERS3 positively regulates ULCFA. Thus, we reveal that the two key players in ULC CER production in epidermis, CERS3 and ELOVL4, are coordinately regulated at both the transcriptional and enzymatic levels.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 3.0 Unported
PISSN	1932-6203
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0067317