Antiviral activity of stachyflin on influenza A viruses of different hemagglutinin subtypes

Motohashi, Yurie; Igarashi, Manabu; Okamatsu, Masatoshi; Noshi, Takeshi; Sakoda, Yoshihiro; Yamamoto, Naoki; Ito, Kimihito; Yoshida, Ryu; Kida, Hiroshi

Antiviral activity of stachyflin on influenza A viruses of different hemagglutinin subtypes

Motohashi, Yurie; Igarashi, Manabu; Okamatsu, Masatoshi et al.

Virology Journal, 2013, 10, 118

Permalink : https://hdl.handle.net/2115/53181

このアイテムのアクセス数:1,138件（2026-10-15 07:29 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
1743-422X-10-118	pdf	1.23 MB	926

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/53181
タイトル	Antiviral activity of stachyflin on influenza A viruses of different hemagglutinin subtypes
著者	Motohashi, Yurie ; Igarashi, Manabu ; Okamatsu, Masatoshi ; Noshi, Takeshi ; Sakoda, Yoshihiro ; Yamamoto, Naoki ; Ito, Kimihito ; Yoshida, Ryu ; Kida, Hiroshi
著者名 Motohashi, Yurie
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
著者名 Noshi, Takeshi
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
著者名 Yamamoto, Naoki
e-Rad_Researcher 60396314 著者名 Ito, Kimihito
著者名 Yoshida, Ryu
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
言語	英語
キーワード	Stachyflin
	Anti-influenza drug
	HA inhibitor
	Docking model
発行日	2013-04-16
出版者	BioMed Central
収録物名	Virology Journal
巻(号)	10
ページ	118
抄録	Background: The hemagglutinin (HA) of influenza viruses is a possible target for antiviral drugs because of its key roles in the initiation of infection. Although it was found that a natural compound, Stachyflin, inhibited the growth of H1 and H2 but not H3 influenza viruses in MDCK cells, inhibitory activity of the compound has not been assessed against H4-H16 influenza viruses and the precise mechanism of inhibition has not been clarified. Methods: Inhibitory activity of Stachyflin against H4-H16 influenza viruses, as well as H1-H3 viruses was examined in MDCK cells. To identify factors responsible for the susceptibility of the viruses to this compound, Stachyflin-resistant viruses were selected in MDCK cells and used for computer docking simulation. Results: It was found that in addition to antiviral activity of Stachyflin against influenza viruses of H1 and H2 subtypes, it inhibited replication of viruses of H5 and H6 subtypes, as well as A(H1N1)pdm09 virus in MDCK cells. Stachyflin also inhibited the virus growth in the lungs of mice infected with A/WSN/1933 (H1N1) and A/chicken/Ibaraki/1/2005 (H5N2). Substitution of amino acid residues was found on the HA2 subunit of Stachyflin-resistant viruses. Docking simulation indicated that D37, K51, T107, and K121 are responsible for construction of the cavity for the binding of the compound. In addition, 3-dimensional structure of the cavity of the HA of Stachyflin-susceptible virus strains was different from that of insusceptible virus strains. Conclusion: Antiviral activity of Stachyflin was found against A(H1N1) pdm09, H5, and H6 viruses, and identified a potential binding pocket for Stachyflin on the HA. The present results should provide us with useful information for the development of HA inhibitors with more effective and broader spectrum.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 2.0 Generic
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/1743-422X-10-118