The PB2, PA, HA, NP, and NS genes of a highly pathogenic avian influenza virus A/whooper swan/Mongolia/3/2005 (H5N1) are responsible for pathogenicity in ducks

Kajihara, Masahiro; Sakoda, Yoshihiro; Soda, Kosuke; Minari, Kenji; Okamatsu, Masatoshi; Takada, Ayato; Kida, Hiroshi

The PB2, PA, HA, NP, and NS genes of a highly pathogenic avian influenza virus A/whooper swan/Mongolia/3/2005 (H5N1) are responsible for pathogenicity in ducks

Kajihara, Masahiro; Sakoda, Yoshihiro; Soda, Kosuke et al.

Virology Journal, 2013, 10, 45

Permalink : https://hdl.handle.net/2115/53253

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アクセス権	open access
URI	https://hdl.handle.net/2115/53253
タイトル	The PB2, PA, HA, NP, and NS genes of a highly pathogenic avian influenza virus A/whooper swan/Mongolia/3/2005 (H5N1) are responsible for pathogenicity in ducks
著者	Kajihara, Masahiro ; Sakoda, Yoshihiro ; Soda, Kosuke ; Minari, Kenji ; Okamatsu, Masatoshi ; Takada, Ayato ; Kida, Hiroshi
e-Rad_Researcher 70711894 著者名 Kajihara, Masahiro
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
著者名 Soda, Kosuke
著者名 Minari, Kenji
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
e-Rad_Researcher 10292062 著者名 Takada, Ayato
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
言語	英語
キーワード	H5N1 influenza virus
	Duck
	Natural host
	Pathogenicity
発行日	2013-02-02
出版者	BioMed Central
収録物名	Virology Journal
巻(号)	10
ページ	45
抄録	Background: Wild ducks are the natural hosts of influenza A viruses. Duck influenza, therefore, has been believed inapparent infection with influenza A viruses, including highly pathogenic avian influenza viruses (HPAIVs) in chickens. In fact, ducks experimentally infected with an HPAIV strain, A/Hong Kong/483/1997 (H5N1) (HK483), did not show any clinical signs. Another HPAIV strain, A/whooper swan/Mongolia/3/2005 (H5N1) (MON3) isolated from a dead swan, however, caused neurological dysfunction and death in ducks. Method: To understand the mechanism whereby MON3 shows high pathogenicity in ducks, HK483, MON3, and twenty-four reassortants generated between these two H5N1 viruses were compared for their pathogenicity in domestic ducks. Results: None of the ducks infected with MON3-based single-gene reassortants bearing the PB2, NP, or NS gene segment of HK483 died, and HK483-based single-gene reassortants bearing PB2, NP, or NS genes of MON3 were not pathogenic in ducks, suggesting that multiple gene segments contribute to the pathogenicity of MON3 in ducks. All the ducks infected with the reassortant bearing PB2, PA, HA, NP, and NS gene segments of MON3 died within five days post-inoculation, as did those infected with MON3. Each of the viruses was assessed for replication in ducks three days post-inoculation. MON3 and multi-gene reassortants pathogenic in ducks were recovered from all of the tissues examined and replicated with high titers in the brains and lungs. Conclusion: The present results indicate that multigenic factors are responsible for efficient replication of MON3 in ducks. In particular, virus growth in the brain might correlate with neurological dysfunction and the disease severity.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-ShareAlike 2.1 Japan
PISSN	1743-422X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/1743-422X-10-45