Identification of the recognition sequence and target proteins for DJ-1 protease

Mitsugi, Hitomi; Niki, Takeshi; Takahashi-Niki, Kazuko; Tanimura, Kyoko; Yoshizawa-Kumagaye, Kumiko; Tsunemi, Masahiko; Iguchi-Ariga, Sanae M. M.; Ariga, Hiroyoshi

Identification of the recognition sequence and target proteins for DJ-1 protease

Mitsugi, Hitomi; Niki, Takeshi; Takahashi-Niki, Kazuko et al.

Febs letters, 2013, 587(16), 2493-2499

Permalink : https://hdl.handle.net/2115/53264

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アクセス権	open access
URI	https://hdl.handle.net/2115/53264
タイトル	Identification of the recognition sequence and target proteins for DJ-1 protease
著者	Mitsugi, Hitomi ; Niki, Takeshi ; Takahashi-Niki, Kazuko ; Tanimura, Kyoko ; Yoshizawa-Kumagaye, Kumiko ; Tsunemi, Masahiko ; Iguchi-Ariga, Sanae M. M. ; Ariga, Hiroyoshi
著者名 Mitsugi, Hitomi
e-Rad_Researcher 90377193 著者名 Niki, Takeshi
e-Rad_Researcher 50447645 著者名 Takahashi-Niki, Kazuko
著者名 Tanimura, Kyoko
著者名 Yoshizawa-Kumagaye, Kumiko
著者名 Tsunemi, Masahiko
e-Rad_Researcher 90184283 著者名 Iguchi-Ariga, Sanae M. M.
e-Rad_Researcher 20143505 著者名 Ariga, Hiroyoshi
言語	英語
キーワード	DJ-1
	Protease
	Biochemistry
発行日	2013-08-19
出版者	Elsevier science bv
収録物名	Febs letters
巻(号)	587(16)
ページ	2493-2499
抄録	DJ-1, the product of familial Parkinson's disease gene and an oncogene, is a cysteine protease which plays a role in anti-oxidative stress reaction. In this study, we identified the recognition sequence for DJ-1 protease by using recombinant DJ-1 and a peptide library. Protease activity of DJ-1 lacking C-terminal alpha-helix (DJ-1 Delta H9) was stronger than that of full-sized DJ-1, and the most susceptible sequence digested by DJ-1 Delta H9 was valine-lysine-valine-alanine (VKVA) under the optimal conditions of pH 5.5 and 0 mM NaCl. Divalent ions, especially Cu2+, were inhibitory to DJ-1's protease activity. c-abl oncogene 1 product (ABL1) and kinesin family member 1B (KIF1B) containing VKVA were digested by DJ-1 Delta H9. Structured summary of protein interactions: DJ-1 cleaves IUF1B by enzymatic study (View interaction) DJ-1 cleaves ABLI by enzymatic study (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
関連情報	PMID 23831022
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2013.06.032