Ca2+-binding properties and regulatory roles of lobster troponin C sites II and IV

Tanaka, Hiroyuki; Takahashi, Hiroki; Ojima, Takao

Febs letters, 2013, 587(16), 2612-2616

Permalink : https://hdl.handle.net/2115/53273

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ファイル	フォーマット	サイズ	閲覧回数	説明
Manuscript	pdf	346 KB	1,093

アクセス権	open access
URI	https://hdl.handle.net/2115/53273
タイトル	Ca2+-binding properties and regulatory roles of lobster troponin C sites II and IV
著者	Tanaka, Hiroyuki ; Takahashi, Hiroki ; Ojima, Takao
e-Rad_Researcher 90241372 著者名 Tanaka, Hiroyuki
e-Rad_Researcher 40283585 著者名 Takahashi, Hiroki
e-Rad_Researcher 30160865 著者名 Ojima, Takao
言語	英語
キーワード	Troponin
	Muscle
	Ca2+-regulation
	Invertebrate
	Arthropod
	Isoform
発行日	2013-08-19
出版者	Elsevier science bv
収録物名	Febs letters
巻(号)	587(16)
ページ	2612-2616
抄録	Invertebrate troponin C typically contains Ca2+-specific binding sites, sites II and IV, in the N- and C-terminal domains, respectively. To investigate the roles of these sites for Ca2+-dependent regulation of muscle contraction, we generated lobster troponin C mutants, and analyzed their Ca2+-binding properties and regulatory effects on actomyosin-tropomyosin Mg-ATPase activity. The results suggest that Ca2+ binding to site IV is responsible for regulation at relatively low Ca2+ concentrations, while site II has an essential role in full activation at higher Ca2+ concentrations. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
NCID	AA00642943
関連情報	PMID 23831063
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2013.06.042