Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species

Ikenaka, Yoshinori; Oguri, Mami; Saengtienchai, Aksorn; Nakayama, Shouta M. M.; Ijiri, Shigeho; Ishizuka, Mayumi

Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species

Ikenaka, Yoshinori; Oguri, Mami; Saengtienchai, Aksorn et al.

Environmental Toxicology and Pharmacology, 2013, 36(2), 567-578

Permalink : https://hdl.handle.net/2115/53434

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アクセス権	open access
URI	https://hdl.handle.net/2115/53434
タイトル	Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species
著者	Ikenaka, Yoshinori ; Oguri, Mami ; Saengtienchai, Aksorn ; Nakayama, Shouta M. M. ; Ijiri, Shigeho ; Ishizuka, Mayumi
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Oguri, Mami
著者名 Saengtienchai, Aksorn
e-Rad_Researcher 90647629 著者名 Nakayama, Shouta M. M.
e-Rad_Researcher 90425421 著者名 Ijiri, Shigeho
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	Fish
	Pyrene
	Conjugation
	Polycyclic aromatic hydrocarbons (PAHs)
	Metabolism
	Inter-species difference
発行日	2013-09
出版者	Elsevier
収録物名	Environmental Toxicology and Pharmacology
巻(号)	36(2)
ページ	567-578
抄録	Metabolic activity, particularly conjugation, was examined in fish by analyzing pyrene (a four-ring, polycyclic aromatic hydrocarbon) metabolites using high-performance liquid chromatography (HPLC) with fluorescence detector (FD), a mass spectrometry (MS) system, and kinetic analysis of conjugation enzymes. Fourteen fresh water fish species, including Danio rerio and Orizias latipes, were exposed to aqueous pyrene, and the resulting metabolites were collected. Identification of pyrene metabolites by HPLC/FD and ion-trap MS indicated that the major metabolites were pyrene glucuronide and pyrene sulfate in all 14 species. Differences were observed in pyrene glucuronide:pyrene sulfate ratio and in the total amount of pyrene conjugates excreted between fish species. Furthermore, a correlation was found between the amount of pyrene glucuronide present and the total amount of the pyrene metabolite eliminated. Kinetic analysis of conjugation by hepatic microsomes in vitro indicated that the differences in excreted metabolites reflected the differences in enzymatic activities. (C) 2013 Elsevier B.V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1382-6689
関連情報	PMID 23834960
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.etap.2013.05.018