Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.

Yanagida, Takashi; Tsushima, Jun; Kitamura, Yoshihisa; Yanagisawa, Daijiro; Takata, Kazuyuki; Shibaike, Tomonori; Yamamoto, Atsuko; Taniguchi, Takashi; Yasui, Hiroyuki; Taira, Takahiro; Morikawa, Shigehiro; Inubushi, Toshihiro; Tooyama, Ikuo; Ariga, Hiroyoshi

Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.

Yanagida, Takashi; Tsushima, Jun; Kitamura, Yoshihisa et al.

Oxidative medicine and cellular longevity, 2009, 2(1), 36-42

Permalink : https://hdl.handle.net/2115/53702

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アクセス権	open access
URI	https://hdl.handle.net/2115/53702
タイトル	Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.
著者	Yanagida, Takashi ; Tsushima, Jun ; Kitamura, Yoshihisa ; Yanagisawa, Daijiro ; Takata, Kazuyuki ; Shibaike, Tomonori ; Yamamoto, Atsuko ; Taniguchi, Takashi ; Yasui, Hiroyuki ; Taira, Takahiro ; Morikawa, Shigehiro ; Inubushi, Toshihiro ; Tooyama, Ikuo ; Ariga, Hiroyoshi
著者名 Yanagida, Takashi
著者名 Tsushima, Jun
e-Rad_Researcher 60195295 著者名 Kitamura, Yoshihisa
著者名 Yanagisawa, Daijiro
e-Rad_Researcher 10434664 著者名 Takata, Kazuyuki
著者名 Shibaike, Tomonori
著者名 Yamamoto, Atsuko
e-Rad_Researcher 10111957 著者名 Taniguchi, Takashi
e-Rad_Researcher 20278443 著者名 Yasui, Hiroyuki
e-Rad_Researcher 70197036 著者名 Taira, Takahiro
e-Rad_Researcher 60220042 著者名 Morikawa, Shigehiro
著者名 Inubushi, Toshihiro
e-Rad_Researcher 20207533 著者名 Tooyama, Ikuo
e-Rad_Researcher 20143505 著者名 Ariga, Hiroyoshi
言語	英語
キーワード	DJ-1
	release
	astrocytes
	focal ischemia
	oxidative stress sensor
	neuroprotection
発行日	2009-01
出版者	Hindawi Pub.
収録物名	Oxidative medicine and cellular longevity
巻(号)	2(1)
ページ	36-42
抄録	Astrocytes, one of the predominant types of glial cells, function as both supportive and metabolic cells for the brain. Under cerebral ischemia/reperfusion-induced oxidative conditions, astrocytes accumulate and activate in the ischemic region. DJ-1 has recently been shown to be a sensor of oxidative stress in living cells. However, the function of astrocytic DJ-1 is still unknown. In the present study, to clarify the effect of astrocytic DJ-1 protein under massive oxidative insult, we used a focal ischemic rat model that had been subjected to middle cerebral artery occlusion (MCAO) and reperfusion. We then investigated changes in the distribution of DJ-1 in astrocytes, DJ-1 release from cultured astrocytes, and the effects of recombinant DJ-1 protein on hydrogen peroxide (H(2)O(2))-induced death in normal and DJ-1-knockdown SH-SY5Y cells and on in vitro scavenging of hydroxyl radicals (()OH) by electron spin resonance spectrometry. At 24 h after 2-h MCAO and reperfusion, an infarct lesion was markedly observed using magnetic resonance imaging and 2,3,5-triphenyltetrazolium chloride staining. In addition, reactive astrocytes enhanced DJ-1 expression in the penumbral zone of the ischemic core and that DJ-1 protein was extracellularly released from astrocytes by H2O2 in in vitro primary cultures. Although DJ-1-knockdown SH-SY5Y cells were markedly vulnerable to oxidative stress, treatment with glutathione S-transferase-tagged recombinant human DJ-1 protein (GST-DJ-1) significantly inhibited H(2)O(2)-induced cell death. In addition, GST-DJ-1 protein directly scavenged ()OH. These results suggest that oxidative stress induces the release of astrocytic DJ-1 protein, which may contribute to astrocyte-mediated neuroprotection.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1942-0994
関連情報	PMID 20046643
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.4161/oxim.2.1.7985