Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses

Miura, Takayuki; Sano, Daisuke; Suenaga, Atsushi et al.

Journal of Virology, 2013, 87(17), 9441-9451

Permalink : https://hdl.handle.net/2115/54031

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アクセス権
open access
URI
https://hdl.handle.net/2115/54031
タイトル
Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses
著者
Miura, Takayuki ; Sano, Daisuke ; Suenaga, Atsushi ; Yoshimura, Takeshi ; Fuzawa, Miyu ; Nakagomi, Toyoko ; Nakagomi, Osamu ; Okabe, Satoshi
著者名
Miura, Takayuki
e-Rad_Researcher
80550368
著者名
Sano, Daisuke
著者名
Suenaga, Atsushi
著者名
Yoshimura, Takeshi
著者名
Fuzawa, Miyu
著者名
Nakagomi, Toyoko
著者名
Nakagomi, Osamu
著者名
Okabe, Satoshi
言語
英語
発行日
2013-06-26
出版者
American Society for Microbiology
収録物名
Journal of Virology
巻(号)
87(17)
ページ
9441-9451
抄録
Histo-blood group antigens (HBGAs) have been suggested to be receptors or coreceptors for human noroviruses (HuNoVs) expressed on the intestinal epithelium. We isolated an enteric bacterium strain (SENG-6), closely related to Enterobacter cloacae, bearing HBGA-like substances from a fecal sample of a healthy individual by using a biopanning technique with anti-HBGA antibodies. The binding capacities of four genotypes of norovirus-like particles (NoVLPs) to Enterobacter sp. SENG-6 cells were confirmed by enzyme-linked immunosorbent assay (ELISA). Transmission electron microscopy demonstrated that NoVLPs bound mainly to extracellular polymeric substances (EPS) of Enterobacter sp. SENG-6, where the HBGA-like substances were localized. EPS that contained HBGA-like substances extracted from Enterobacter sp. SENG-6 was shown by enzyme-linked immunosorbent assay (ELISA) to be capable of binding to NoVLPs of a GI.1 wild-type strain (8fIIa) and a GII.6 strain that can recognize A antigen but not to an NoVLP GI.1 mutant strain (W375A) that loses the ability to bind to A antigen. Enzymatic cleavage of terminal N-acetyl-galactosamine residues in the bacterial EPS weakened bacterial EPS binding to the GI.1 wild-type strain (8fIIa). These results indicate that A-like substances in the bacterial EPS play a key role in binding to NoVLPs. Since the specific binding of HuNoVs to HBGA-positive enteric bacteria is likely to affect the transmission and infection processes of HuNoVs in their hosts and in the environment, further studies of human enteric bacteria and their binding capacity to HuNoVs will provide a new scientific platform for understanding interactions between two types of microbes that were previously regarded as biologically unrelated.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0022-538X
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1128/JVI.01060-13