Renibacterium salmoninarum の表在抗原p57をELISA用抗原とした抗体検出

antibody-detection ELISA

We evaluated p57 of Renibacterium salmoninarum (R.s.) as a capture antigen for specific antibody-detection ELISA against R.s.. Using formalin-killed R.s. (FK-R.s.) as ELISA antigens, ELISA values (OD492 (nm)) of the negative control sera showed high values ranging from 0.12 to 1.59. These values were relatively lower than those of sera from the fish immunized with FK-R.s. (0.16-2.6), but seemed to be still high as negative control sera. Contrarily, when p57 was used as a capture antigen, ELISA values of the negative control sera were all 0.1 or less, while those of the sera from immunized fish ranged from 0.02 to 1.28. It was thus demonstrated that high background of ELISA was enabled to be reduced by p57 as ELISA antigens.