Transcriptional regulation of the legumain gene by p53 in HCT116 cells

Yamane, Takuya; Murao, Sato; Kato-Ose, Izumi; Kashima, Lisa; Yuguchi, Motoki; Kozuka, Miyuki; Takeuchi, Keisuke; Ogita, Hisakazu; Ohkubo, Iwao; Ariga, Hiroyoshi

Transcriptional regulation of the legumain gene by p53 in HCT116 cells

Yamane, Takuya; Murao, Sato; Kato-Ose, Izumi et al.

Biochemical and biophysical research communications, 2013, 438(4), 613-618

Permalink : https://hdl.handle.net/2115/54547

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アクセス権	open access
URI	https://hdl.handle.net/2115/54547
タイトル	Transcriptional regulation of the legumain gene by p53 in HCT116 cells
著者	Yamane, Takuya ; Murao, Sato ; Kato-Ose, Izumi ; Kashima, Lisa ; Yuguchi, Motoki ; Kozuka, Miyuki ; Takeuchi, Keisuke ; Ogita, Hisakazu ; Ohkubo, Iwao ; Ariga, Hiroyoshi
著者名 Yamane, Takuya
著者名 Murao, Sato
著者名 Kato-Ose, Izumi
e-Rad_Researcher 30404750 著者名 Kashima, Lisa
著者名 Yuguchi, Motoki
著者名 Kozuka, Miyuki
e-Rad_Researcher 40432306 著者名 Takeuchi, Keisuke
e-Rad_Researcher 50379236 著者名 Ogita, Hisakazu
e-Rad_Researcher 80152073 著者名 Ohkubo, Iwao
e-Rad_Researcher 20143505 著者名 Ariga, Hiroyoshi
言語	英語
キーワード	Legumain
	p53
	Doxorubicin
発行日	2013-09-06
出版者	Academic press inc elsevier science
収録物名	Biochemical and biophysical research communications
巻(号)	438(4)
ページ	613-618
抄録	Legumain (EC 3.4.22.34) is an asparaginyl endopeptidase. Strong legumain activity was observed in the mouse kidney, and legumain was found to be highly expressed in tumors. We previously reported that bovine kidney annexin A2 was co-purified with legumain and that legumain cleaved the N-terminal region of annexin A2 at an Asn residue in vitro and in vivo. In this study, we found a p53-binding site in intron 1 of the human legumain gene using computational analysis. To determine whether transcription of the legumain gene is regulated by p53, HCT116 cells were transfected with p53 siRNA and the effect of knockdown of p53 expression on legumain expression was examined. The results showed that expression levels of both legumain mRNA and protein were decreased in the siRNA-treated cells. Furthermore, enzyme activity of legumain was also increased by doxorubicin and its activity was reduced by knockdown of p53 in HCT116 cells. These results suggest that legumain expression and its enzyme activity are regulated by p53. (C) 2013 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 23942113
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2013.08.007