open access

https://hdl.handle.net/2115/54727

Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy

英語

2013-03

Mary Ann Liebert

Photomedicine and Laser Surgery

31(3)

1-7

Objective: The objective of this research was to determine the effectiveness of antimicrobial photodynamic therapy (aPDT) in the removal of mycoplasmas from contaminated cells. Background data: Mycoplasmas often contaminate cell cultures. The cell-contaminating mycoplasmas are removed by antibiotics, but the use of antibiotics usually induces antibiotic-resistant bacteria. aPDT is expected to be a possible alternative to antibiotic treatments for suppressing infections. Materials and Methods: Mycoplasma salivarium (Ms)-infected human embryonic kidney (HEK) 293 cells were irradiated using a red light-emitting diode (LED) in the presence of methylene blue (MB) as a photosensitizer. The Ms viable count was determined using culture on agar plates or using a mycoplasma detection kit. Results: aPDT performed using red LED irradiation was effective in decreasing live Ms in the presence of MB without damaging the HEK293 cells. aPDT removed live Ms from the infected cells after washing the cells with sterilized phosphate-buffered saline (PBS) to decrease the initial number of live Ms before aPDT. Conclusions: This study suggests that aPDT could remove mycoplasmas from contaminated cells.

学術雑誌論文

Version of Record

This is a copy of an article published in the Photomedicine and Laser Surgery © 2013 copyright Mary Ann Liebert, Inc.; Photomedicine and Laser Surgery is available online at: http://online.liebertpub.com.

1549-5418

1557-8550

AA11968895