Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction

Kurasaki, Masaaki; Sun, Yongkun; Komori, Miyako; Miyajima, Miki; Hosokawa, Toshiyuki; Saito, Takeshi

Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction

Kurasaki, Masaaki; Sun, Yongkun; Komori, Miyako et al.

Advances in Biological Chemistry, 2012, 2(3), 243-247

Permalink : https://hdl.handle.net/2115/54753

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アクセス権	open access
URI	https://hdl.handle.net/2115/54753
タイトル	Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction
著者	Kurasaki, Masaaki ; Sun, Yongkun ; Komori, Miyako ; Miyajima, Miki ; Hosokawa, Toshiyuki ; Saito, Takeshi
e-Rad_Researcher 80161727 著者名 Kurasaki, Masaaki
著者名 Sun, Yongkun
著者名 Komori, Miyako
著者名 Miyajima, Miki
e-Rad_Researcher 00157025 著者名 Hosokawa, Toshiyuki
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
言語	英語
キーワード	DNA Damage
	Quantification
	TUNEL
	Apoptosis
	PC12 Cells
発行日	2012-08
出版者	Scientific Research Publishing Inc.
収録物名	Advances in Biological Chemistry
巻(号)	2(3)
ページ	243-247
抄録	An improved terminal deoxynucleotidyl transferase- mediated dUTP-biotin nick end labeling method for the quantification of DNA damage in tissues and cultured cells was developed. Many reports have revealed that histochemistry of DNA damage can be visualized using immunohistochemistry for the terminal deoxynucleotidyl transferase reaction in tissue sections. However, few reports have described quantification of DNA damage in tissues or cells. In this study, to estimate the degree of DNA damage, the confirmed method for histochemistry using biotinylated dUTP and deoxynucleotidyl transferase was applied to label the cleaved DNA ends caused by DNA damage in tissues or cells. After end-labeling, avidin-conjugated peroxidase was reacted. A significant correlation was observed between numbers of cleaved DNA ends and peroxidase activity after the reaction. The obtained signals for presented method showed higher than those for ordinary method, and correlate with degree of DNA damage caused by serum deprivation and chemical dose. In addition, DNA damage caused by apoptosis in cells treated with 6-hydroxydopamine or Cu and in the tissues of rats administered a diet containing no Zn could be evaluated quantitatively using the present method.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 3.0 Unported
権利情報	Copyright © 2012 by authors and Scientific Research Publishing Inc.
PISSN	2162-2183
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.4236/abc.2012.23030