Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins

Ohno, Kaoru; Sawada, Jun-ichi; Takiya, Shigeharu; Kimoto, Mai; Matsumoto, Akiko; Tsubota, Takuya; Uchino, Keiro; Hui, Chi-chung; Sezutsu, Hideki; Handa, Hiroshi; Suzuki, Yoshiaki

Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins

Ohno, Kaoru; Sawada, Jun-ichi; Takiya, Shigeharu et al.

Journal of biological chemistry, 2013, 288(44), 31581-31591

Permalink : https://hdl.handle.net/2115/54948

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アクセス権	open access
URI	https://hdl.handle.net/2115/54948
タイトル	Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins
著者	Ohno, Kaoru ; Sawada, Jun-ichi ; Takiya, Shigeharu ; Kimoto, Mai ; Matsumoto, Akiko ; Tsubota, Takuya ; Uchino, Keiro ; Hui, Chi-chung ; Sezutsu, Hideki ; Handa, Hiroshi ; Suzuki, Yoshiaki
e-Rad_Researcher 10260035 著者名 Ohno, Kaoru
著者名 Sawada, Jun-ichi
e-Rad_Researcher 50179587 著者名 Takiya, Shigeharu
著者名 Kimoto, Mai
著者名 Matsumoto, Akiko
著者名 Tsubota, Takuya
著者名 Uchino, Keiro
著者名 Hui, Chi-chung
e-Rad_Researcher 70342805 著者名 Sezutsu, Hideki
e-Rad_Researcher 80107432 著者名 Handa, Hiroshi
e-Rad_Researcher 50132733 著者名 Suzuki, Yoshiaki
言語	英語
キーワード	Gene transcription
	Homeobox
	Silkworm
	Tissue-specific transcription factors
	Transcription enhancers
	Fibroin Gene
	Homeodomain
	Silk gland factor-2 (SGF-2)
発行日	2013-11-01
出版者	Amer soc biochemistry molecular biology inc
収録物名	Journal of biological chemistry
巻(号)	288(44)
ページ	31581-31591
抄録	SGF-2 binds to promoter elements governing posterior silk gland-specific expression of the fibroin gene in Bombyx mori. Wepurified SGF-2 and showed that SGF-2 contains at least four gene products: the silkworm orthologues of LIM homeodomain protein Awh, LIM domain-binding protein (Ldb), a sequence-specific single-stranded DNA-binding protein (Lcaf), and the silk protein P25/fibrohexamerin (fhx). Using co-expression of these factors in Sf9 cells, Awh, Ldb, and Lcaf proteins were co-purified as a ternary complex that bound to the enhancer sequence in vitro. Lcaf interacts with Ldb as well as Awh through the conserved regions to mediate transcriptional activation in yeast. Misexpression of Awh in transgenic silkworms induces ectopic expression of the fibroin gene in the middle silk glands, where Ldb and Lcaf are expressed. Taken together, this study demonstrates that SGF-2 is a multisubunit activator complex containing Awh. Moreover, our results suggest that the Ldb.Lcaf protein complex serves as a scaffold to facilitate communication between transcriptional control elements.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This research was originally published in Journal of Biological Chemistry. Shigeharu Takiya(s). Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins. Journal of Biological Chemistry. Year;2013 Vol:288(44)pp31518-pp31519. Copylight ©2013 the American Society for Biochemistry and Molecular Biology.
PISSN	0021-9258
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1074/jbc.M113.514471