open access

https://hdl.handle.net/2115/55124

Relationship between neutrophil influx and oxidative stress in alveolar space in lipopolysaccharide-induced lung injury

英語

Lung injury model

2014-01-15

Elsevier science bv

Respiratory physiology & neurobiology

191

75-83

We intratracheally administered lipopolysaccharide (LPS) to ICR mice and then collected BAL fluid and lung tissue to determine whether levels of neutrophils and/or myeloperoxidase (MPO) in bronchoalveolar lavage (BAL) fluid reflect lung tissue damage. Robust neutrophil accumulation into the alveolar space and lung tissue were almost completely abolished at seven days along with oxidative stress markers in the lung. However, lung injury scores and lung wet/dry ratios, as well as MPO and oxidative stress markers in BAL fluid were significantly increased at five and seven days after LPS administration. At later time points, BAL neutrophils generated more MPO activity and ROS than those harvested sooner after LPS administration. Although elevated neutrophil levels in BAL fluid reflected oxidative stress in the lungs, MPO might serve as a useful marker to evaluate damage sustained by epithelial cells over the long term. (C) 2013 Elsevier B.V. All rights reserved.

学術雑誌論文

Accepted Manuscript

1569-9048

AA1161265X