Effects of different promoters on the virulence and immunogenicity of a HIV-1 Env-expressing recombinant vaccinia vaccine

Isshiki, Mao; Zhang, Xianfeng; Sato, Hirotaka; Ohashi, Takashi; Inoue, Makoto; Shida, Hisatoshi

Effects of different promoters on the virulence and immunogenicity of a HIV-1 Env-expressing recombinant vaccinia vaccine

Isshiki, Mao; Zhang, Xianfeng; Sato, Hirotaka et al.

Vaccine, 2014, 32(7), 839-845

Permalink : https://hdl.handle.net/2115/55256

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アクセス権	open access
URI	https://hdl.handle.net/2115/55256
タイトル	Effects of different promoters on the virulence and immunogenicity of a HIV-1 Env-expressing recombinant vaccinia vaccine
著者	Isshiki, Mao ; Zhang, Xianfeng ; Sato, Hirotaka ; Ohashi, Takashi ; Inoue, Makoto ; Shida, Hisatoshi
著者名 Isshiki, Mao
e-Rad_Researcher 40374681 著者名 Zhang, Xianfeng
著者名 Sato, Hirotaka
e-Rad_Researcher 10282774 著者名 Ohashi, Takashi
著者名 Inoue, Makoto
e-Rad_Researcher 00144395 著者名 Shida, Hisatoshi
言語	英語
キーワード	LC16m8 Delta
	Promoter pSFJ1-10
	Promoter p7.5
	HIV-1 Env
	Immunogenicity
	Safety
発行日	2014-02-07
出版者	Elsevier sci ltd
収録物名	Vaccine
巻(号)	32(7)
ページ	839-845
抄録	Previously, we developed a vaccination regimen that involves priming with recombinant vaccinia virus LC16m8 Delta (rm8 Delta) strain followed by boosting with a Sendai virus-containing vector. This protocol induced both humoral and cellular immune responses against the HIV-1 envelope protein. The current study aims to optimize this regimen by comparing the immunogenicity and safety of two rm8 Delta strains that express HIV-1 Env under the control of a moderate promoter, p7.5, or a strong promoter, pSFJ1-10. m8 Delta-p7.5-JRCSFenv synthesized less gp160 but showed significantly higher growth potential than m8 Delta-pSFJ-JRCSFenv. The two different rm8 Delta strains induced antigen-specific immunity; however, m8 Delta-pSFJ-JRCSFenv elicited a stronger anti-Env antibody response whereas m8 Delta-p7.5-JRCSFenv induced a stronger Env-specific cytotoxic T lymphocyte response. Both strains were less virulent than the parental m8 Delta strain, suggesting that they would be safe for use in humans. These findings indicate the vaccine can be optimized to induce favorable immune responses (either cellular or humoral), and forms the basis for the rational design of an AIDS vaccine using recombinant vaccinia as the delivery vector. (C) 2013 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0264-410X
NCID	AA10491877
関連情報	PMID 24370703
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.vaccine.2013.12.022