Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain

Asano, Nozomi; Atsuumi, Haruka; Nakamura, Akiyoshi; Tanaka, Yoshikazu; Tanaka, Isao; Yao, Min

Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain

Asano, Nozomi; Atsuumi, Haruka; Nakamura, Akiyoshi et al.

Biochemical and Biophysical Research Communications, 2014, 443(4), 1251-1256

Permalink : https://hdl.handle.net/2115/55598

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アクセス権	open access
URI	https://hdl.handle.net/2115/55598
タイトル	Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain
著者	Asano, Nozomi ; Atsuumi, Haruka ; Nakamura, Akiyoshi ; Tanaka, Yoshikazu ; Tanaka, Isao ; Yao, Min
著者名 Asano, Nozomi
著者名 Atsuumi, Haruka
著者名 Nakamura, Akiyoshi
著者名 Tanaka, Yoshikazu
e-Rad_Researcher 70093052 著者名 Tanaka, Isao
e-Rad_Researcher 40311518 著者名 Yao, Min
言語	英語
キーワード	Ribosome biogenesis
	Interaction
	Intrinsically disordered protein
	Isothermal titration calorimetty
	Circular dichroism
発行日	2014-01-24
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	443(4)
ページ	1251-1256
抄録	Removal of anti-association factor, Tif6 (eIF6), by elongation factor-like 1 (EFL1) and Shwachman-Bodian-Diamond syndrome (SBDS) protein is a critical step in the late stage of ribosome maturation. Although EFL1 is known to have GTPase activity that is stimulated by SBDS, how they cooperatively trigger dissociation of Tif6 from the ribosome remains to be elucidated. In the present study, the interaction between EFL1 and SBDS was analyzed by size exclusion chromatography, gel shift assay, and isothermal titration calorimetry (ITC). The results showed that EFL1 interacted directly with SBDS. ITC experiments using domain-truncated mutants showed that the interaction between EFL1 and SBDS is governed by the insertion domain of EFL1 and domains II-III of SBDS. Circular dichroism spectroscopy showed that the insertion domain of EFL1 has a random structure in the absence of SBDS, whereas the disadvantageous entropy change observed on ITC suggested a fixed conformation coupled with complex formation with SBDS. Based on these observations together with those reported previously, we propose roles of EFL1 and SBDS in ribosomal maturation. (C) 2014 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 24406167
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2013.12.143