open access

https://hdl.handle.net/2115/56117

スラリー埋没加熱処理にアパタイト薄膜形成がチタン表面の骨形成に及ぼす影響 [全文の要約]

英語

Titanium

2014-03-25

Hokkaido University

https://www.lib.hokudai.ac.jp/dissertations/copy-guides/

The aim of this study was to evaluate the effects of the surface treatment of titanium(Ti) with thin apatite (Ap) coating by the chemical coating process using calcium phosphate slurry on the osteoconductivity by in vivo and in vitro study. Ti wires (1 mm in diameter and 8 mm in length) and Ti discs (21.5mm in diameter) were buried in calcium-phosphate slurry, and subsequently heated at 973 K in air (CP-coated Ti). Annealed Ti wires and discs, which were prepared by heating at 973 K in air without the slurry, were used as control(AnnealTi). On the surface of Ti, thin coating film consisted Ap and TiO2 with 300 nm in thickness was observed by SEM and XPS. To evaluate osteoconductivity,CP-coated Ti and Anneal Ti wires were implanted in rat femora, and histological investigation and histomorphometric evaluation was done at 2 and 8 weeks after surgery. B one marrow stromal cells in rats were cultivated on CP-coated Ti and Anneal Ti discs. The cells were then cultured for 2 and 3 weeks, and ALP activity standardized by DNA contents, osteopontin (OP), osteocalcin (OC) and calcium contents were measured biochemically. RT-PCR was done for collagen I and Cbfa1 after cultured for 1 and 2 weeks. At 2 weeks after surgery, immature bone tissue including many osteocytes was observed around both specimens. At 8 weeks, newly formed bone on the surface of the both specimens was remodeled and thickness of it decreased. The area of the specimen directly attached to bone tissue was significantly extensive in CPcoated Ti than in Anneal Ti at both 2 and 8 weeks. In vitro study, at 2 weeks, OC and calcium contents of CP-coated Ti were significantly higher than those of Anneal Ti. At 3 weeks, all parameters of CP-coated Ti were significantly higher than those of Anneal Ti. RT-PCR showed more expression of mRNA encoding Cbfa1 of CP-coated Ti at 1 week. It was suggested that thin Ap film by the chemical coating process was effective to enhance the osteoconductivity of Ti.

甲第11242号

博士(歯学)

(主査) 教授 横山 敦郎, 教授 網塚 憲生, 教授 土門卓文, 准教授 飯塚 正

歯学研究科（口腔医学専攻）

博士論文

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