open access

https://hdl.handle.net/2115/56241

高気孔性PLGA /β-TCPスキャフォールドとFGF2による骨増生効果 [全文の要約]

英語

regenerative scaffold

2014-03-25

Hokkaido University

https://www.lib.hokudai.ac.jp/dissertations/copy-guides/

β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) and fibroblast growth factor-2 (FGF2) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the PLGA/FGF2-coated β-TCP scaffold on bone augmentation. The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture and a subcutaneous implant test. Subsequently, the β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffold loaded with FGF2, were implanted into rat cranial bone. Histological observations were taken at 10 and 35 days post-surgery. SEM and TEM observations showed a thin PLGA layer on the β-TCP particle after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater compared to the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples from bone implanted with PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation, suggesting that FGF2-loaded PLGA/β-TCP scaffold was expected for bone tissue engineering.

甲第11263号

博士(歯学)

(主査) 教授 川浪 雅光, 教授 田村 正人, 教授 網塚 憲生

歯学研究科（口腔医学専攻）

博士論文

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