The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.

Kang, Sung-Sik; Ofuji, Sosuke; Imai, Kei; Huang, Weiping; Koyama, Keisuke; Yanagawa, Yojiro; Takahashi, Yoshiyuki; Nagano, Masashi

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.

Kang, Sung-Sik; Ofuji, Sosuke; Imai, Kei et al.

Zygote, 2014, 23(3), 412-415

Permalink : https://hdl.handle.net/2115/56333

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アクセス権	open access
URI	https://hdl.handle.net/2115/56333
タイトル	The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.
著者	Kang, Sung-Sik ; Ofuji, Sosuke ; Imai, Kei ; Huang, Weiping ; Koyama, Keisuke ; Yanagawa, Yojiro ; Takahashi, Yoshiyuki ; Nagano, Masashi
著者名 Kang, Sung-Sik
著者名 Ofuji, Sosuke
e-Rad_Researcher 70343994 著者名 Imai, Kei
著者名 Huang, Weiping
著者名 Koyama, Keisuke
著者名 Yanagawa, Yojiro
e-Rad_Researcher 70167485 著者名 Takahashi, Yoshiyuki
e-Rad_Researcher 70312402 著者名 Nagano, Masashi
言語	英語
キーワード	Bovine
	Embryonic Development
	Droplet
	IVC
	WOW
発行日	2014-05
出版者	Cambridge University Press
収録物名	Zygote
巻(号)	23(3)
ページ	412-415
抄録	The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	© Cambridge University Press 2014
PISSN	1469-8730
NCID	AA10958262
関連情報	PMID 24598303
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1017/S096719941400001X