Prematurational culture with 3-isobutyl-1-methylxanthine synchronizes meiotic progression of the germinal vesicle stage and improves nuclear maturation and embryonic development in in vitro-grown bovine oocytes.

Huang, Weiping; Nagano, Masashi; Kang, Sung-Sik; Yanagawa, Yojiro; Takahashi, Yoshiyuki

Prematurational culture with 3-isobutyl-1-methylxanthine synchronizes meiotic progression of the germinal vesicle stage and improves nuclear maturation and embryonic development in in vitro-grown bovine oocytes.

Huang, Weiping; Nagano, Masashi; Kang, Sung-Sik et al.

The Journal of reproduction and development, 2014, 60(1), 9-13

Permalink : https://hdl.handle.net/2115/56342

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アクセス権	open access
URI	https://hdl.handle.net/2115/56342
タイトル	Prematurational culture with 3-isobutyl-1-methylxanthine synchronizes meiotic progression of the germinal vesicle stage and improves nuclear maturation and embryonic development in in vitro-grown bovine oocytes.
著者	Huang, Weiping ; Nagano, Masashi ; Kang, Sung-Sik ; Yanagawa, Yojiro ; Takahashi, Yoshiyuki
著者名 Huang, Weiping
e-Rad_Researcher 70312402 著者名 Nagano, Masashi
著者名 Kang, Sung-Sik
著者名 Yanagawa, Yojiro
e-Rad_Researcher 70167485 著者名 Takahashi, Yoshiyuki
言語	英語
キーワード	Early antral follicle
	Fertilization
	Germinal vesicle
	IBMX
	Pre-IVM culture
発行日	2014-02
出版者	日本繁殖生物学会
収録物名	The Journal of reproduction and development
巻(号)	60(1)
ページ	9-13
抄録	The objective of this study was to clarify the effects of prematurational culture (pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95 μm in diameter) derived from early antral follicles (0.5-1 mm in diameter) were cultured for 12 days for in vitro growth (IVG). IVG oocytes with a normal appearance were subjected to examinations of diameter and chromatin structure in the germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about 115 μm. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2, the fertilizability and developmental competence of IVG oocytes were examined. Regardless of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but were lower than that of in vivo-grown oocytes (88.0%). Cleavage and blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with pre-IVM was lower than that of in vivo-grown oocytes. In conclusion, pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes, probably due to promotion of their chromatin transition and synchronization of meiotic progression.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1348-4400
NCID	AA10936678
関連情報	PMID 24212477
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2013-082