Identification of the predominant nonrestoring allele for Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.): development of molecular markers for the maintainer genotype

Moritani, Mari; Taguchi, Kazunori; Kitazaki, Kazuyoshi; Matsuhira, Hiroaki; Katsuyama, Takaya; Mikami, Tetsuo; Kubo, Tomohiko

Identification of the predominant nonrestoring allele for Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.): development of molecular markers for the maintainer genotype

Moritani, Mari; Taguchi, Kazunori; Kitazaki, Kazuyoshi et al.

Molecular Breeding, 2013, 32(1), 91-100

Permalink : https://hdl.handle.net/2115/56480

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アクセス権	open access
URI	https://hdl.handle.net/2115/56480
タイトル	Identification of the predominant nonrestoring allele for Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.): development of molecular markers for the maintainer genotype
著者	Moritani, Mari ; Taguchi, Kazunori ; Kitazaki, Kazuyoshi ; Matsuhira, Hiroaki ; Katsuyama, Takaya ; Mikami, Tetsuo ; Kubo, Tomohiko
著者名 Moritani, Mari
著者名 Taguchi, Kazunori
著者名 Kitazaki, Kazuyoshi
著者名 Matsuhira, Hiroaki
著者名 Katsuyama, Takaya
e-Rad_Researcher 50133715 著者名 Mikami, Tetsuo
e-Rad_Researcher 40261333 著者名 Kubo, Tomohiko
言語	英語
キーワード	Marker-assisted selection
	Restorer of fertility
	Genetic association
	PCR marker
	Multi-allelic locus
発行日	2013-06
出版者	springer
収録物名	Molecular Breeding
巻(号)	32(1)
ページ	91-100
抄録	Hybrid seed production in sugar beet relies on cytoplasmic male sterility (CMS). As time-consuming and laborious test crosses with a CMS tester are necessary to identify maintainer lines, development of a marker-assisted selection method for the rf gene (the nonrestoring allele of restorer-of-fertility locus) is highly desirable for sugar-beet breeding. To develop such a method, we investigated genetic variation at the Rf1 locus, one of two Rf loci known in sugar beet. After HindIII-digestion, genomic DNAs from beet plants known to have a restoring Rf1 allele yielded a range of hybridization patterns on agarose gels, indicating that Rf1 is a multi-allelic locus. However, the hybridization patterns of 22 of 23 maintainer lines were indistinguishable. The nucleotide sequences of the rf1 coding regions of these 22 maintainer lines were found to be identical, confirming that the lines had the same rf1 allele. Two PCR markers were developed that targeted a downstream intergenic sequence and an intron of Rf1. The electrophoretic patterns of both markers indicated multiple Rf1 alleles, one of which, named the dd(L) type, was associated with the maintainer genotype. To test the validity of marker-assisted selection, 147 sugar beet plants were genotyped using these markers. Additionally, the 147 sugar beet plants were crossed with CMS plants to determine whether they possessed the maintainer genotype. Analysis of 5038 F1 offspring showed that 53 % of the dd(L) plants, but none of the plants with other alleles, had the maintainer genotype. Thus, selection for the dd(L) type considerably enriched the proportion of plants with the maintainer genotype.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	1380-3743
EISSN	1572-9788
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11032-013-9854-8