Possible involvement of insulin-like growth factor 2 mRNA-binding protein 3 in zebrafish oocyte maturation as a novel cyclin B1 mRNA-binding protein that represses the translation in immature oocytes

Takahashi, Kazuki; Kotani, Tomoya; Katsu, Yoshinao; Yamashita, Masakane

Possible involvement of insulin-like growth factor 2 mRNA-binding protein 3 in zebrafish oocyte maturation as a novel cyclin B1 mRNA-binding protein that represses the translation in immature oocytes

Takahashi, Kazuki; Kotani, Tomoya; Katsu, Yoshinao et al.

Biochemical and Biophysical Research Communications, 2014, 448(1), 22-27

Permalink : https://hdl.handle.net/2115/56598

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アクセス権	open access
URI	https://hdl.handle.net/2115/56598
タイトル	Possible involvement of insulin-like growth factor 2 mRNA-binding protein 3 in zebrafish oocyte maturation as a novel cyclin B1 mRNA-binding protein that represses the translation in immature oocytes
著者	Takahashi, Kazuki ; Kotani, Tomoya ; Katsu, Yoshinao ; Yamashita, Masakane
著者名 Takahashi, Kazuki
e-Rad_Researcher 70419852 著者名 Kotani, Tomoya
e-Rad_Researcher 00332180 著者名 Katsu, Yoshinao
e-Rad_Researcher 30202378 著者名 Yamashita, Masakane
言語	英語
キーワード	CPEB
	IMP3
	Pumilio
	MPF
	mRNA localization
	Translational control
発行日	2014-05-23
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	448(1)
ページ	22-27
抄録	In immature zebrafish oocytes, dormant cyclin B1 mRNAs localize to the animal polar cytoplasm as aggregates. After hormonal stimulation, cyclin B1 mRNAs are dispersed and translationally activated, which are necessary and sufficient for the induction of zebrafish oocyte maturation. Besides cytoplasmic polyadenylation element-binding protein (CPEB) and cis-acting elements in the 3' untranslated region (UTR), Pumilio1 and a cis-acting element in the coding region of cyclin B1 mRNA are important for the subcellular localization and timing of translational activation of the mRNA. However, mechanisms underlying the spatio-temporal control of cyclin B1 mRNA translation during oocyte maturation are not fully understood. We report that insulin-like growth factor 2 mRNA-binding protein 3 (IMP3), which was initially described as a protein bound to Vg1 mRNA localized to the vegetal pole of Xenopus oocytes, binds to the 3' UTR of cyclin B1 mRNA that localizes to the animal pole of zebrafish oocytes. IMP3 and cyclin B1 riaRNA co-localize to the animal polar cytoplasm of immature oocytes, but in mature oocytes, IMP3 dissociates from the mRNA despite the fact that its protein content and phosphorylation state are unchanged during oocyte maturation. IMP3 interacts with Pumiliol and CPEB in an mRNA-dependent manner in immature oocytes but not in mature oocytes. Overexpression of IMP3 and injection of anti-IMP3 antibody delayed the progression of oocyte maturation. On the basis of these results, we propose that IMP3 represses the translation of cyclin B1 mRNA in immature zebrafish oocytes and that its release from the mRNA triggers the translational activation.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	(C) 2014 Elsevier Inc. All rights reserved.
PISSN	0006-291X
関連情報	PMID 24735541
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2014.04.020