Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide

Miyaji, Hirofumi; Kanayama, Izumi; Takita, Hiroko; Nishida, Erika; Tsuji, Maiko; Fugetsu, Bunshi; Sun, Ling; Inoue, Kana; Ibara, Asako; Akasaka, Tsukasa; Sugaya, Tsutomu; Kawanami, Masamitsu

Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide

Miyaji, Hirofumi; Kanayama, Izumi; Takita, Hiroko et al.

International Journal of Nanomedicine, 2014, 9, 3363-3373

Permalink : https://hdl.handle.net/2115/56661

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アクセス権	open access
URI	https://hdl.handle.net/2115/56661
タイトル	Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide
著者	Miyaji, Hirofumi ; Kanayama, Izumi ; Takita, Hiroko ; Nishida, Erika ; Tsuji, Maiko ; Fugetsu, Bunshi ; Sun, Ling ; Inoue, Kana ; Ibara, Asako ; Akasaka, Tsukasa ; Sugaya, Tsutomu ; Kawanami, Masamitsu
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
著者名 Kanayama, Izumi
e-Rad_Researcher 30125330 著者名 Takita, Hiroko
著者名 Nishida, Erika
著者名 Tsuji, Maiko
e-Rad_Researcher 40281844 著者名 Fugetsu, Bunshi
著者名 Sun, Ling
著者名 Inoue, Kana
著者名 Ibara, Asako
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
e-Rad_Researcher 10211301 著者名 Sugaya, Tsutomu
e-Rad_Researcher 10133761 著者名 Kawanami, Masamitsu
言語	英語
キーワード	GO
	RGO
	biomaterials
	cell ingrowth
	regenerative scaffold
	tissue engineering
発行日	2014-07-11
出版者	Dove Medical Press
収録物名	International Journal of Nanomedicine
巻(号)	9
ページ	3363-3373
抄録	Graphene oxide (GO) is a single layer carbon sheet with a thickness of less than 1 nm. GO has good dispersibility due to surface modifications with numerous functional groups. Reduced graphene oxide (RGO) is produced via the reduction of GO, and has lower dispersibility. We examined the bioactivity of GO and RGO films, and collagen scaffolds coated with GO and RGO. METHODS:GO and RGO films were fabricated on a culture dish. Some GO films were chemically reduced using either ascorbic acid or sodium hydrosulfite solution, resulting in preparation of RGO films. The biological properties of each film were evaluated by scanning electron microscopy (SEM), atomic force microscopy, calcium adsorption tests, and MC3T3-E1 cell seeding. Subsequently, GO- and RGO-coated collagen scaffolds were prepared and characterized by SEM and compression tests. Each scaffold was implanted into subcutaneous tissue on the backs of rats. Measurements of DNA content and cell ingrowth areas of implanted scaffolds were performed 10 days post-surgery. RESULTS: The results show that GO and RGO possess different biological properties. Calcium adsorption and alkaline phosphatase activity were strongly enhanced by RGO, suggesting that RGO is effective for osteogenic differentiation. SEM showed that RGO-modified collagen scaffolds have rough, irregular surfaces. The compressive strengths of GO- and RGO-coated scaffolds were approximately 1.7-fold and 2.7-fold greater, respectively, when compared with the non-coated scaffold. Tissue ingrowth rate was 39% in RGO-coated scaffolds, as compared to 20% in the GO-coated scaffold and 16% in the non-coated scaffold. CONCLUSION: In summary, these results suggest that GO and RGO coatings provide different biological properties to collagen scaffolds, and that RGO-coated scaffolds are more bioactive than GO-coated scaffolds.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 3.0 United States
PISSN	1176-9114
EISSN	1178-2013
NCID	AA1215861X
関連情報	PMID 25050063
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2147/IJN.S62342