Hyaluronic acid enhances the effect of the PAMPS/PDMAAm double-network hydrogel on chondrogenic differentiation of ATDC5 cells

Kitamura, Nobuto; Kurokawa, Takayuki; Fukui, Takaaki; Gong, Jian P.; Yasuda, Kazunori

Hyaluronic acid enhances the effect of the PAMPS/PDMAAm double-network hydrogel on chondrogenic differentiation of ATDC5 cells

Kitamura, Nobuto; Kurokawa, Takayuki; Fukui, Takaaki et al.

BMC Musculoskeletal Disorders, 2014, 15, 222

Permalink : https://hdl.handle.net/2115/56867

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アクセス権	open access
URI	https://hdl.handle.net/2115/56867
タイトル	Hyaluronic acid enhances the effect of the PAMPS/PDMAAm double-network hydrogel on chondrogenic differentiation of ATDC5 cells
著者	Kitamura, Nobuto ; Kurokawa, Takayuki ; Fukui, Takaaki ; Gong, Jian P. ; Yasuda, Kazunori
e-Rad_Researcher 80447044 著者名 Kitamura, Nobuto
e-Rad_Researcher 40451439 著者名 Kurokawa, Takayuki
著者名 Fukui, Takaaki
e-Rad_Researcher 20250417 著者名 Gong, Jian P.
e-Rad_Researcher 20166507 著者名 Yasuda, Kazunori
言語	英語
キーワード	Hyaluronic acid
	Chondrogenic differentiation
	ATDC5
	Double-network hydrogel
	Polymer
発行日	2014-07-06
出版者	Biomed Central
収録物名	BMC Musculoskeletal Disorders
巻(号)	15
ページ	222
抄録	Background: A double-network (DN) gel, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N,N'-dimethyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo. The present study investigated whether DN gel induced chondrogenic differentiation of ATDC5 cells in a maintenance medium without insulin, and whether supplementation of hyaluronic acid enhanced the chondrogenic differentiation effect of DN gel. Methods: ATDC5 cells were cultured on the DN gel and the polystyrene (PS) dish in maintenance media without insulin for 21 days. Hyaluronic acid having a molecular weight of approximately 800 kDa was supplemented into the medium so that the concentration became 0.01, 0.1, or 1.0 mg/mL. The cultured cells were evaluated using immunocytochemistry for type-2 collagen and real time PCR for gene expression of type-2 collagen, aggrecan, and Sox9 at 7 and 21 days of culture. Results: The cells cultured on the DN gel formed nodules and were stained with an anti-type-2 collagen antibody, and expression of type-2 collagen and aggrecan mRNA was significantly greater on the DN gel than on the PS dish surface (p < 0.05) in the hyaluronic acid-free maintenance medium. Hyaluronic acid supplementation of a high concentration (1.0 mg/mL) significantly enhanced expression of type-2 collagen and aggrecan mRNA in comparison with culture without hyaluronic acid at 21 days (p < 0.05). Conclusions: The DN gel induced chondrogenic differentiation of ATDC5 cells without insulin. This effect was significantly affected by hyaluronic acid, depending on the level of concentration. There is a high possibility that hyaluronic acid plays an important role in the in vivo hyaline cartilage regeneration phenomenon induced by the DN gel.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 2.0 Generic
PISSN	1471-2474
関連情報	PMID 24997593
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/1471-2474-15-222