open access

https://hdl.handle.net/2115/57048

La importancia de los límites de detección en el diagnóstico de la forunculosis asintomática

スペイン語

detection limits

2014

Veterinaria Organización

Revista Electronica de Veterinaria

15(6)

1-11

The success of the methods employed in the detection of Aeromonas salmonicida depends on several factors including detection limits which are not always considered when planning a diagnostic protocol. First, detection limits of A. salmonicida by Coomassie Brilliant Blue Agar (CBBA) culture, Immuno-Fluorescence Antibody Test (IFAT), Coagglutination test (CoAgT) and Polymerase Chain Reaction (PCR) were analyzed, then a controlled wet-lab experiment was conducted using 60 masu salmon (Oncorhynchus masou) fry inoculated with A. salmonicida and based on the results obtained, an epizootiological survey of selected rivers in Hokkaido was then conducted. Detection limits were 10^2 for CBBA, 10^4 for the IFAT, 10^5 for PCR and 10^3 for the CoAgT from an original de A. salmonicida broth culture of 1.3 x 10^8 CFU/mL demonstrating that CBBA and the CoAgT were better at detecting the bacteria at low concentrations. The wet-lab analysis produced results of detection limits of a similar trend, with CBBA, IFAT, PCR and CoAgT at 10^-3, 10^-3, 10^-1 and 10^-3 from kidney samples of 9.9 X 10^4 CFU/g respectively. The results from the epizootiological survey in Hokkaido showed positive from CBBA with IFAT, CoAgT and PCR corroborating them in varying degrees thus demonstrating the importance of a multi-diagnostic approach in scenarios of this nature.

学術雑誌論文

Version of Record

1695-7504