Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel

Ishida, Sumire; Tanaka, Ryosuke; Yamaguchi, Naoya et al.

PLOS ONE, 2014, 9(8), e99655

Permalink : https://hdl.handle.net/2115/57093

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アクセス権
open access
URI
https://hdl.handle.net/2115/57093
タイトル
Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel
著者
Ishida, Sumire ; Tanaka, Ryosuke ; Yamaguchi, Naoya ; Ogata, Genki ; Mizutani, Takeomi ; Kawabata, Kazushige ; Haga, Hisashi
著者名
Ishida, Sumire
著者名
Tanaka, Ryosuke
著者名
Yamaguchi, Naoya
著者名
Ogata, Genki
e-Rad_Researcher
40451405
著者名
Mizutani, Takeomi
著者名
Kawabata, Kazushige
e-Rad_Researcher
00292045
著者名
Haga, Hisashi
言語
英語
発行日
2014-08-29
出版者
Public Library of Science
収録物名
PLOS ONE
巻(号)
9(8)
ページ
e99655
抄録
Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-beta 1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-beta 1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
権利情報
Creative Commons Attribution 4.0 International
PISSN
1932-6203
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1371/journal.pone.0099655