Nanoparticulation of BCG-CWS for application to bladder cancer therapy

Nakamura, Takashi; Fukiage, Masafumi; Higuchi, Megumi; Nakaya, Akihiro; Yano, Ikuya; Miyazaki, Jun; Nishiyama, Hiroyuki; Akaza, Hideyuki; Ito, Toshihiro; Hosokawa, Hiroyuki; Nakayama, Toshinori; Harashima, Hideyoshi

Nanoparticulation of BCG-CWS for application to bladder cancer therapy

Nakamura, Takashi; Fukiage, Masafumi; Higuchi, Megumi et al.

Journal of controlled release, 2014, 176, 44-53

Permalink : https://hdl.handle.net/2115/57253

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アクセス権	open access
URI	https://hdl.handle.net/2115/57253
タイトル	Nanoparticulation of BCG-CWS for application to bladder cancer therapy
著者	Nakamura, Takashi ; Fukiage, Masafumi ; Higuchi, Megumi ; Nakaya, Akihiro ; Yano, Ikuya ; Miyazaki, Jun ; Nishiyama, Hiroyuki ; Akaza, Hideyuki ; Ito, Toshihiro ; Hosokawa, Hiroyuki ; Nakayama, Toshinori ; Harashima, Hideyoshi
著者名 Nakamura, Takashi
著者名 Fukiage, Masafumi
著者名 Higuchi, Megumi
e-Rad_Researcher 60301149 著者名 Nakaya, Akihiro
e-Rad_Researcher 60047008 著者名 Yano, Ikuya
e-Rad_Researcher 10550246 著者名 Miyazaki, Jun
e-Rad_Researcher 20324642 著者名 Nishiyama, Hiroyuki
e-Rad_Researcher 70010486 著者名 Akaza, Hideyuki
著者名 Ito, Toshihiro
e-Rad_Researcher 60431756 著者名 Hosokawa, Hiroyuki
e-Rad_Researcher 50237468 著者名 Nakayama, Toshinori
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
言語	英語
キーワード	Packaging
	Nanoparticle
	Delivery system
	Bladder cancer
	BCG-CWS
	Cancer immunotherapy
発行日	2014-02-28
出版者	Elsevier science bv
収録物名	Journal of controlled release
巻(号)	176
ページ	44-53
抄録	The Mycobacterium bovis Bacille Calmette-Guerin cell wall skeleton (BCG-CWS) could be used to replace live BCG as a bladder cancer drug. However, because BCG-CWS is poorly soluble, has a strong-negative charge, very high molecular weight and heterogeneity in size of tens of mu m, it cannot be used in such an application. We report herein on the development of a novel packaging method that permits BCG-CWS to be encapsulated into 166 nm-sized lipid particles. The BCG-CWS encapsulated nano particle (CWS-NP) has a high uniformity and can be easily dispersed. Thus, it has the potential for use as a packaging method that would advance the scope of applications of BCG-CWS as a bladder cancer drug. In a functional evaluation, CWS-NP was efficiently taken up by mouse bladder tumor (MBT-2) cells in vitro and inhibited tumor growth in mice bearing MBT-2 tumors. Moreover, intravesically administered CWS-NP showed significant antitumor effects in a rat model with naturally developed bladder cancer. An enhancement in Th1 differentiation by CWS-NP was also confirmed in human T cells. In conclusion, CWS-NP represents a promising delivery system for BCG-CWS for clinical development as a potent bladder cancer drug. (C) 2013 Elsevier B. V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0168-3659
関連情報	PMID 24389133
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jconrel.2013.12.027