A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

Yamada, Yuma; Tabata, Mai; Yasuzaki, Yukari; Nomura, Masatoshi; Shibata, Atsushi; Ibayashi, Yuta; Taniguchi, Yosuke; Sasaki, Shigeki; Harashima, Hideyoshi

A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line

Yamada, Yuma; Tabata, Mai; Yasuzaki, Yukari et al.

Biomaterials, 2014, 35(24), 6430-6438

Permalink : https://hdl.handle.net/2115/57456

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アクセス権	open access
URI	https://hdl.handle.net/2115/57456
タイトル	A nanocarrier system for the delivery of nucleic acids targeted to a pancreatic beta cell line
著者	Yamada, Yuma ; Tabata, Mai ; Yasuzaki, Yukari ; Nomura, Masatoshi ; Shibata, Atsushi ; Ibayashi, Yuta ; Taniguchi, Yosuke ; Sasaki, Shigeki ; Harashima, Hideyoshi
e-Rad_Researcher 60451431 著者名 Yamada, Yuma
著者名 Tabata, Mai
著者名 Yasuzaki, Yukari
e-Rad_Researcher 30315080 著者名 Nomura, Masatoshi
著者名 Shibata, Atsushi
著者名 Ibayashi, Yuta
e-Rad_Researcher 00452714 著者名 Taniguchi, Yosuke
e-Rad_Researcher 10170672 著者名 Sasaki, Shigeki
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
言語	英語
キーワード	MIN6 cell
	Nucleic acids delivery
	Multifunctional envelope-type nano device (MEND)
	Pancreatic beta cells
	Diabetes
	microRNA
発行日	2014-08
出版者	Elsevier
収録物名	Biomaterials
巻(号)	35(24)
ページ	6430-6438
抄録	Pancreatic beta cells secrete insulin in response to glucose levels and thus are involved in controlling blood glucose levels. A line of pancreatic beta cells "MIN6" has been used in studies related to the function of beta cells and diabetes therapy. Regulating gene expression in MIN6 cells could accelerate these studies, but an efficient method for the transfection of nucleic acids targeted to MINE cells is required. We report here on a liposome-based carrier targeted to pancreatic beta cells (Multifunctional envelope-type nano device for pancreatic beta cells, beta-MEND). We identified a lipid composition for use in preparing the beta-MEND, which permits the particles to be efficiently internalized into MIN6, as evidenced by flow cytometry analyses. Intracellular observation by confocal laser scanning microscopy showed that the beta-MEND efficiently delivered the oligo nucleic acids to the cytosol of MINE cells. Moreover, using a beta-MEND encapsulating a 2'-O-Methyl RNA complementary to a microRNA that suppresses insulin secretion, the knockdown of the targeted microRNA and an up-regulation of insulin secretion were observed in MINE. Thus, the beta-MEND holds promise as an efficient system for delivering nucleic acids targeted to MIN6 and can contribute to research and therapy aimed at diabetes. (C) 2014 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0142-9612
関連情報	PMID 24816283
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.biomaterials.2014.04.017