Gene expression profiling of Pseudomonas putida F1 after exposure to aromatic hydrocarbon in soil by using proteome analysis

Morimoto, Hajime; Kuwano, Masayoshi; Kasahara, Yasuhiro

Gene expression profiling of Pseudomonas putida F1 after exposure to aromatic hydrocarbon in soil by using proteome analysis

Morimoto, Hajime; Kuwano, Masayoshi; Kasahara, Yasuhiro

Archives of Microbiology, 2013, 195(12), 805-813

Permalink : https://hdl.handle.net/2115/57643

このアイテムのアクセス数:807件（2026-08-25 17:19 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
AM_Kasahara	pdf	1.70 MB	1,045
AM_Kasahara_FS1	pdf	539 KB	403	Supplementary Material Fig. S1
AM_Kasahara_TS1	pdf	29.6 KB	375	Supplementary Material Table S1
AM_Kasahara_TS2	pdf	42.5 KB	430	Supplementary Material Table S2
AM_Kasahara_TS3	pdf	279 KB	715	Supplementary Material Table S3
AM_Kasahara_TS4	pdf	173 KB	1,030	Supplementary Material Table S4

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/57643
タイトル	Gene expression profiling of Pseudomonas putida F1 after exposure to aromatic hydrocarbon in soil by using proteome analysis
著者	Morimoto, Hajime ; Kuwano, Masayoshi ; Kasahara, Yasuhiro
著者名 Morimoto, Hajime
著者名 Kuwano, Masayoshi
e-Rad_Researcher 20273849 著者名 Kasahara, Yasuhiro
言語	英語
キーワード	Proteome analysis
	aromatic hydrocarbon
	soil
	in situ
	Pseudomonas putida
	bacteria
発行日	2013-12
出版者	Springer Berlin / Heidelberg
収録物名	Archives of Microbiology
巻(号)	195(12)
ページ	805-813
抄録	Pseudomonas putida F1 can metabolize toluene, ethylbenzene, and benzene for growth. Previously, we identified proteins involved in the utilization of these compounds by P. putida F1 through culture in liquid media. However, it was unclear whether laboratory analysis of bacterial activity and catabolism accurately reflected the soil environment. We identified proteins involved in the degradation of toluene, ethylbenzene, and benzene growth in soil using two-dimensional gel electrophoresis (2-DE) or standard SDS-PAGE combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS). According to 2-DE/LC–MS/MS analysis, 12 of 22 key enzymes involved in the degradation of toluene, ethylbenzene, and benzene were detected. In standard SDS-PAGE/LC–MS/MS analysis of soil with ethylbenzene, approximately 1,260 cellular proteins were identified in P. putida F1. All key enzymes and transporter and sensor proteins involved in ethylbenzene degradation were up-regulated similar to that noted in liquid cultures. In P. putida F1, aromatic hydrocarbon response in soil is the same as that observed in liquid media.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0302-8933
関連情報	PMID 24136143
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00203-013-0932-4